Registration Dossier

Administrative data

Endpoint:
toxicity to terrestrial arthropods: short-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: non GLP screening study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report Date:
2016

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
no
Application method:
oral

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
- Controls: No
- Chemical name of vehicle: 25 % DMSO and 75 % water
- Evaporation of vehicle before use: No

Test organisms

Test organisms (species):
other: Myzus persicae
Animal group:
other: Sternorrhyncha
Details on test organisms:
TEST ORGANISM
- Common name: green peach aphid
- Disease free: yes
- Kept according to standard practices: yes

Study design

Study type:
laboratory study
Limit test:
no
Total exposure duration:
3 d

Test conditions

Test temperature:
23 ± 1 °C
Humidity:
50 ± 5 %
Details on test conditions:
TEST SYSTEM
- Test container: 96-well-microtiter plate
- No. of organisms per container (treatment): 5 - 8
- No. of replicates per treatment group: 4
- No. of replicates per control / vehicle control: 0

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality and fecundity

VEHICLE CONTROL PERFORMED: no

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3
Nominal and measured concentrations:
nominal: 0.003, 0.01, 0.03, 0.1, 0.316, 1, 3.16, 10, 31.62 and 100 mg/L
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Key result
Duration:
5 d
Dose descriptor:
other: ED50
Effect conc.:
> 100 other: mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
not determinable
Remarks:
No mortality or effects on fecundity were observed.
Details on results:
- Mortality at end of exposure period: No
- Morphological abnormalities: No
- Behavioural abnormalities: No
Reported statistics and error estimates:
Applied doses and corresponding mortality rating data were submitted to a 2-parameter non-linear regression using SAS 9.4 (SAS, Cary, North Carolina).

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Remarks:
Only screening study