Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Remarks:
Screening study
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2014-12-02 to 2014-12-04
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Only basic data from a screening study available, no analytical monitoring was conducted.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014
Report Date:
2014

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Lot/batch No.of test material: Lot 003

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
100.1 mg of the test substance were added to 900 mL M4-media and stirred for 4 hours. Than the stock solution was filled up to 1L. The stock solution appeared milky turbid and the pH was 8.0 (correspond to test concentration of 100 mg/L). The pH of M4 media was 8.0.
Dilution solution 1:10 mL of the stock solution was diluted with M4 media to 100 mL (correspond to test concentration of 10 mg/L). Dilution solution 2:10 mL of the dilution solution 1 was diluted with M4 media to 100 mL (correspond to test concentration of 1 mg/L).

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna (Water flea)
- Strain/clone: STRAUS
- Source: The clone of Daphnia magna was bred at the testing facility.
- Age of parental stock: Juvenile daphnids (< 24 hours)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
No data
Test temperature:
No data
pH:
Within the ranges required by the OECD Guideline for Testing of Chemicals No. 202
Dissolved oxygen:
Within the ranges required by the OECD Guideline for Testing of Chemicals No. 202
Conductivity:
No data
Nominal and measured concentrations:
Nominal: 0 (control, solvent control), 1, 10 and 100 mg/L.
No measured concentrations.
Details on test conditions:
TEST SYSTEM
- Fill volume: 10 mL test volume
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 1

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Immobilisation of daphnids is determined after 24 and 48 hours of exposure.

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Conclusions:
After 48 hours an EC50 of >100 mg/L was observed (based on nominal concentrations).
Executive summary:

The effect of the test substance on the immobilisation of Daphnia magna STRAUS was investigated in a screening study. Juvenile daphnids (<24 h old) were exposed to 1, 10 and 100 mg/L (nominal) of the test substance and compared to a control. Analytical monitoring was not conducted. Immobilisation of the daphnids is determined after 24 and 48 hours of exposure. After 48 hours an EC50 of >100 mg/L was observed (based on nominal concentrations).