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Ecotoxicological information

Short-term toxicity to fish

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Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
significant methodological deficiencies
Principles of method if other than guideline:
A study was conducted to determine the short term toxicity of the test substance to fish according to an unspecified method. Oryzias latipes were exposed for 96 h to the test substance at concentrations of 100, 500 and 1000 mg/L (nominal). Mortality was assessed daily.
GLP compliance:
no
Specific details on test material used for the study:
Batch no.: 40406
Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
Not recorded
Test organisms (species):
Oryzias latipes
Details on test organisms:
- Name: Orange red killifish
- Length and weight: mean body length +/- SD = 23.9 +/- 1.44 mm, mean body weight +/- SD = 0.24 +/- 0.04 g
Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
No
Hardness:
Not recorded
Test temperature:
25 +/- 1°C
pH:
Not recorded
Dissolved oxygen:
Not recorded
Nominal and measured concentrations:
100, 500 and 1000 mg/L (nominal)
Details on test conditions:
10 fish per condition
Test aquarium: Glass made (200 x 265 x 300 mm)
Test volume: 5 L per exposure concentration
Statistics: Probit method (p=0.05)
Reference substance (positive control):
yes
Remarks:
Pentachlorophenol sodium salt
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
>= 100 - <= 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
96 h LC50 = 0.37 (0.32 - 0.42) mg/L
Validity criteria fulfilled:
not specified
Conclusions:
Under the study conditions, the 96 h LC50 of the substance to Oryzias latipes was determined to be greater than 100 mg/L (nominal).
Executive summary:

A study was conducted to determine the short term toxicity of the substance to fish according to an unspecified method. Oryzias latipes were exposed for 96 h to the test substance at concentrations of 100, 500 and 1000 mg/L (nominal). Mortality was assessed daily. Under the study conditions, the 96 h LC50 of the substance to Oryzias latipes was determined to be greater than 100 mg/L (nominal) (Yamada, 1984). The study is considered unsuitable for risk assessment purposes due to lack of information on the impurities of the test substance and on critical experimental data.

Description of key information

Under the read-across study conditions, the mean measured 96 h LC0 of the substance in Danio rerio was determined to be greater than 78 mg/L (measured) of the organic components (equivalent to 95 mg/L of the test substance including the inorganic components). The LC50 could not be determined due to absence of any adverse effects.

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

A study was conducted to determine the short-term toxicity of the read-across substance to Zebra fish according to EU Method C.1, in compliance with GLP. The fish were exposed for 96 h to the test substance at concentrations of 0 and 100 mg/L. Mortality and symptoms (swimming action) were assessed daily. Analytical verification of test concentrations was conducted by TOC determination. There were no mortality or symptoms observed in any group. Under the study conditions, the mean measured 96 h LC0 of the substance in anio rerio was determined to be greater than 78 mg/L of the organic components (equivalent to 95 mg/L of the test substance including inorganic components) (Caspers, 1999). The LC50 could not be determined due to absence of any adverse effects.