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Diss Factsheets
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EC number: 203-467-0 | CAS number: 107-14-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 995
Materials and methods
- Principles of method if other than guideline:
- The assay was performed as described by Jaylet et al. (1986) and according to the recommendations of the French standard AFNOR T-90-325 (AFNOR, 1987) revised in 1992 (AFNOR, 1992).
Briefly, larvae at stage 53 of the developmental table established by Gallien and Durocher (1957) were used for the experiment, since at this stage the mitotic index is at a maximum (Deparis, 1973). The highest concentration to be used in the 12-day micronucleus assay is defined as half the minimum concentration that led to detectable physiological disturbances (weight loss, swelling, reduction in food intake, swimming in circles, etc.) in a 6-day preliminary toxicity test. In every glass container, the water (containing the dissolved chemical at the required concentration) was renewed daily. After 12 days of treatment, blood samples were taken from every animal (15-20 larvae per concentration) by cardiac puncture into an heparinized micropipette. The slides (one for each animal) were stained with Masson acid Hemalun and the number of micronucleated erythocytes was counted in a sample of 1000 erythrocytes. - GLP compliance:
- not specified
- Type of assay:
- other: Newt micronucleus test
Test material
- Reference substance name:
- Chloroacetonitrile
- EC Number:
- 203-467-0
- EC Name:
- Chloroacetonitrile
- Cas Number:
- 107-14-2
- Molecular formula:
- C2H2ClN
- IUPAC Name:
- 2-chloroacetonitrile
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- other: Pleurodeles waltl larvae
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- Test organisms were provided by the Centre de Biologie du Développement, University P. Sabatier (Toulouse, France) and acclimatized for one week before the experiment.
Administration / exposure
- Route of administration:
- other: test organisms in water containing the test substance
- Vehicle:
- water (containing the dissolved chemical at the required concentration)
- Duration of treatment / exposure:
- 12 days
Doses / concentrationsopen allclose all
- Dose / conc.:
- 1.25 other: µg/ml
- Dose / conc.:
- 2.5 other: µg/ml
- Dose / conc.:
- 5 other: µg/ml
- No. of animals per sex per dose:
- 15-20 larvae per concentration
Examinations
- Tissues and cell types examined:
- Blood, erythrocytes
- Details of tissue and slide preparation:
- Blood samples were taken from every animal by cardiac puncture into an heparinized micropipette. The slides were stained with Masson acid Hemalun and the number of micronucleated erythocytes was counted in a sample of 1000 erythrocytes.
- Evaluation criteria:
- A compound is considered as genotoxic if:
(1) it induces a statistically significant increase in the number of micronucleated erythrocytes compared to the control (p < 0.05) and
(2) the median for the treated group is at least twice as high as the control median.
In case the second condition is not fulfilled, the chemical is considered as weakly genotoxic. - Statistics:
- As there is not a normal distribution of micronucleus frequencies, median values and quartiles were calculated instead of mean values. The statistical method of Mac Gill et al. (1978), a quick and reliable test adapted to small sample sizes and values that do not have normal distribution, was used to analyse the results.
Results and discussion
Test results
- Sex:
- not specified
- Genotoxicity:
- positive
- Remarks:
- 1.25-2.5 (clastogenic effects)
- Toxicity:
- yes
- Remarks:
- 10 µg/ml
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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