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Environmental fate & pathways

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Biodegradation in water

28 -days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test item 5-amino-2-chlorobenzenesulphonic acid (CAS No.88-43-7) (Experimental study report, 2018). The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used for this study. 1 polyseed capsule were added in 500 ml D.I water and then stirred for 1 hour for proper mixing and functioning of inoculum. This gave the bacterial count as 10E7 to 10E8 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l . OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 79.51%. Degradation of Sodium Benzoate exceeds 64.46 % on 7 days & 75.30 % on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid.The BOD28 value of test chemical was observed to be 0.72 mgO2/mg. ThOD was calculated as 1 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 72%. Based on the results, the test item, under the test conditions, was considered to be readily biodegradable in nature.

Biodegradation in water and sediment

Estimation Programs Interface (EPI Suite, 2017) prediction model was run to predict the half-life in water and sediment for the test compound 5-amino-2-chlorobenzenesulfonic acid (CAS No. 88 -43 -7). If released in to the environment, 35% of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of 5-amino-2-chlorobenzenesulfonic acid in water is estimated to be 37.5 days (900 hrs). The half-life (37.5 days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is moderate to low whereas the half-life period of 5-amino-2-chlorobenzenesulfonic acid in sediment is estimated to be 337.5 days (8100 hrs). However, as the percentage release of test chemical into the sediment is less than 1% (i.e, reported as 0.0835%), indicates that 5-amino-2-chlorobenzenesulfonic acid is not persistent in sediment.

 

Biodegradation in soil

The half-life period of 5-amino-2-chlorobenzenesulfonic acid (CAS No.88 -43 -7) in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (EPI suite, 2017). If released into the environment, 64.9% of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of 5-amino-2-chlorobenzenesulfonic acid in soil is estimated to be 75 days (1800 hrs). Based on this half-life value of 5-amino-2-chlorobenzenesulfonic acid, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to low.

Additional information

Biodegradation in water

28 -days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test item 5-amino-2-chlorobenzenesulphonic acid (CAS No.88-43-7) (Experimental study report, 2018). The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used for this study. 1 polyseed capsule were added in 500 ml D.I water and then stirred for 1 hour for proper mixing and functioning of inoculum. This gave the bacterial count as 10E7 to 10E8 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l . OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 79.51%. Degradation of Sodium Benzoate exceeds 64.46 % on 7 days & 75.30 % on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid.The BOD28 value of test chemical was observed to be 0.72 mgO2/mg. ThOD was calculated as 1 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 72%. Based on the results, the test item, under the test conditions, was considered to be readily biodegradable in nature.

Biodegradation in water and sediment

Estimation Programs Interface (EPI Suite, 2017) prediction model was run to predict the half-life in water and sediment for the test compound 5-amino-2-chlorobenzenesulfonic acid (CAS No. 88 -43 -7). If released in to the environment, 35% of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of 5-amino-2-chlorobenzenesulfonic acid in water is estimated to be 37.5 days (900 hrs). The half-life (37.5 days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is moderate to low whereas the half-life period of 5-amino-2-chlorobenzenesulfonic acid in sediment is estimated to be 337.5 days (8100 hrs). However, as the percentage release of test chemical into the sediment is less than 1% (i.e, reported as 0.0835%), indicates that 5-amino-2-chlorobenzenesulfonic acid is not persistent in sediment.

 

Biodegradation in soil

The half-life period of 5-amino-2-chlorobenzenesulfonic acid (CAS No.88 -43 -7) in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (EPI suite, 2017). If released into the environment, 64.9% of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of 5-amino-2-chlorobenzenesulfonic acid in soil is estimated to be 75 days (1800 hrs). Based on this half-life value of 5-amino-2-chlorobenzenesulfonic acid, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to low.

On the basis of available information, the test substance 5-amino-2-chlorobenzenesulfonic acid can be considered to be readily biodegradable in nature.