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EC number: 244-435-6 | CAS number: 21544-03-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Endpoint summary
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
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- Additional toxicological data

Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1977
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- no
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Bis(2,3-epoxypropyl) cyclohex-4-ene-1,2-dicarboxylate
- EC Number:
- 244-435-6
- EC Name:
- Bis(2,3-epoxypropyl) cyclohex-4-ene-1,2-dicarboxylate
- Cas Number:
- 21544-03-6
- Molecular formula:
- C14H18O6
- IUPAC Name:
- 1,2-bis[(oxiran-2-yl)methyl] cyclohex-4-ene-1,2-dicarboxylate
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Tif: RAIf (SPF) strain
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Animals
Healthy random bred rats of the Tif: RAIf (SPF) strain raised on our premises were used for these experiments.
They were kept at a room temperature of 22 + 1° C, at a relative humidity of 55 + 5 % and on a 10 hours light cycle day. They received ad libitum rat food - NAFAG, Gossau SG - and water. Prior to treatment the animals were adapted to our laboratories for a
minimum of 4 days and the initial body weight ranged from 180 to 200 grams.
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- Treatment* and observations
During the treatment and observation period the rats were housed individually in Macrolon cages (type 2). Approximately 24 hours before treatment an area on the back of the rats of approximately 60 square cm was shaved with an electric clipper.
For treatment the test material was evenly dispersed on the skin with a syringe and was covered with an occlusive dressing which was fastened around the trunk with an adhesive elastic bandage. After 24 hours the dressing was removed, the skin was
cleaned with lukewarm water and the reaction of the skin was appraised.
*Noakes, D.N. and Sanderson, D.M. A method for determining the dermal toxicity of pesticides, Brit. J. Industr. Med., 26, 59-64, 1969 - Duration of exposure:
- 24 hours
- Doses:
- 3590 and 4640
- No. of animals per sex per dose:
- 5 male and 5 female
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: no weight observed
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, gross pathology, signs and symptomes - Statistics:
- Not applicable
Results and discussion
- Preliminary study:
- Not applicable
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 4 600 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- no indication of skin irritation up to the relevant limit dose level
- Mortality:
- No mortality occured during the test
- Clinical signs:
- Within 24 hours after treatment the rats in all osage groups showed sedation, dyspnoea, curved position and ruffled fur. No local skin irritation was seen.
The animals recovered from systemic symptoms within 8 to 14 days. They were submitted at random to a necropsy at the end of the observation period. - Body weight:
- Not observed
- Gross pathology:
- No substance related gross organ changes were seen.
- Other findings:
- N/A
Any other information on results incl. tables
Rate of death:
Died within | |||||||||||||
Concentration % of Formulation | N° of animals | 1 hour | 24 hours | 48 hours | 7 days | 14 days | |||||||
Dose mg/kg | male | female | male | female | male | female | male | female | male | female | male | female | |
3590 | conc. | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
4640 | conc. | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
No higher doses were possible |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute dermal LD50 of the test item in rats of both sexes observed over a period of 14 days is greater than 4600 mg/kg.
The test material has therefore practically no acute toxicity to the rat by this route of administration. - Executive summary:
The acute dermal LD50 of the test item in rats of both sexes observed over a period of 14 days is greater than 4600 mg/kg.
5 male and 5 females per concentrations were treated at 3590 and 4640 mg/kg with the test item unchanged.
The test site was covered with an occlusive dressing during 24 hours and wascleaned with lukewarm water and the reaction of the skin was appraised.
As no mortality occured during the test, animals were necropsied at the end of 14 days observation period
Signs and symptoms
Within 24 hours after treatment the rats in all dosage groups showed sedation, dyspnoea, curved position and ruffled fur. No local skin irritation was seen.
The animals recovered from systemic symptoms within 8 to 14 days. They were submitted at random to a necropsy at the end of the observation period.
Killed animals: No substance related gross organ changes were seen.
The acute dermal LD50 of the test item in rats of both sexes observed over a period of 14 days is greater than 4600 mg/kg.
The test material has therefore practically no acute toxicity to the rat by this route of administration.
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