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EC number: 263-856-6 | CAS number: 63105-49-7
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: screening test, other
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 2009
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- see Methodology
- GLP compliance:
- not specified
- Remarks:
- field study
Test material
- Reference substance name:
- Trisodium [5-acetamido-4-hydroxy-3-[[2-hydroxy-4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonato(5-)]cuprate(3-)
- EC Number:
- 263-856-6
- EC Name:
- Trisodium [5-acetamido-4-hydroxy-3-[[2-hydroxy-4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonato(5-)]cuprate(3-)
- Cas Number:
- 63105-49-7
- Molecular formula:
- C20H14CuN3O15S4.3Na C20H14CuN3Na3O15S4
- IUPAC Name:
- trisodium [5-acetamido-4-hydroxy-3-[[2-hydroxy-4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonato(5-)]cuprate(3-)
- Test material form:
- liquid - solid: mixture of
- Remarks:
- manufacturing industrial wastewater
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic (adaptation not specified)
- Details on inoculum:
- 1. The Reactive Violet 5R manufacturing industry’s wastewater was collected from Ganesh Dyechem industry, Vatva, Ahmedabad, India. The pH of the wastewater generated after dye synthesis was in the range of 4.0–5.5. The dye manufacturing industry adjusts the pH of the wastewater around neutral before discharging it into the common effluent treatment plant.
2. Soil samples collected from contaminated sites around Ganesh Dyechem Industry situated in Vatva industrial estate, Gujarat, were used for isolation and screening of dye-decolourizing cultures by enrichment culture techniques using nutrient broth amended with 300 mg/L RV5 dye - Duration of test (contact time):
- 8 h
Initial test substance concentrationopen allclose all
- Initial conc.:
- 32 640 mg/L
- Based on:
- COD
- Remarks:
- all organic matter in the industrial wastewater; 1:20 dilution
- Initial conc.:
- > 500 - < 4 000 mg/L
- Based on:
- test mat.
- Remarks:
- dye concentration; 1:20 dilution
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- other: COD reduction
- Parameter followed for biodegradation estimation:
- other: copper remediation
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 95
- Sampling time:
- 8 h
- Remarks on result:
- other: biodegradable in a flow fixed film bioreactor with P. aeruginosa
- Parameter:
- other: COD reduction %
- Value:
- 88
- Sampling time:
- 8 h
- Parameter:
- other: copper remediation %
- Value:
- 98
- Sampling time:
- 8 h
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- other: biodegradable in a down flow fixed film bioreactor
- Conclusions:
- Reactive Violet 5R was assessed for its general biodegradation potential in an experiment with a down flow fixed silm bioreactor (DFFR) containing a culture of Pseudomonas aeruginosa. The wastewater from an industrial site in India where the reactive dye has been manufactured was used for the biodegradation study. The COD of the 1:20 diluted wastewater was >32000 mg/L (containing ca. 500 - 4000 mg/L dye substance). A reduction of ca 88% could be determined for the COD, 95% of the organic matter could be removed by biodegradation, even 99% copper were remediated. The study could show that reactive dyes in wastewater have a certain potential to be biodegradable in special treatments like the DFFR. However, since the experimental design is not reflecting the conditions used in OECD test guidelines, the substance is not considered readily biodegradable.
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