Pyridine-2-thiol 1-oxide, sodium salt

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Pyridine-2-thiol 1-oxide, sodium salt

EC Number:

223-296-5

EC Name:

Pyridine-2-thiol 1-oxide, sodium salt

Cas Number:

3811-73-2

Molecular formula:

C5H5NOS.Na

IUPAC Name:

sodium (1-oxo-1λ⁵-pyridin-2-yl)sulfanide

Details on test material:

- Analytical purity: 40.8%
- Lot/batch No.: 99072150

Method

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with

Metabolic activation system:

S9 mix

Test concentrations with justification for top dose:

Experiment 1: 0; 6.25; 12.5; 25; 50; 100 µg a.s./plate;
Experiment 2, strains TA 1535, TA 98, TA 100, TA 102: 0; 3.13; 6.25; 12.5; 25; 50; 100 µg a.s./plate
Experiment 2, strain TA1537: 0; 1.56; 3.13; 6.25; 12.5; 25; 50 µg a.s./plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: water

Controls

Positive controls:

yes

Remarks:

Sodium azide; 9-Aminoacridine; 2-Nitrofluorene; 2-Aminoanthracene; Cumene hydroperoxide; Dimethylsulfoxide

Details on test system and experimental conditions:

METHOD OF APPLICATION: Experiment 1: plate-incorporation method; Experiment 2: pre-incubation method.
DURATION: Preincubation period: Experiment 1: n.a. Experiment 2: 30 min.

Evaluation criteria:

Number of revertant colonies per plate

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

see remarks

Additional information on results:

The test substance did not induce two-fold increases in the number of revertant colonies in the plate incorporation or pre-incubation assay, at any dose level, in any tester strain, in the absence or presence of S9 metabolism.

Any other information on results incl. tables

Cytotoxicity -

Experiment 1:

100 µg a.s./plate (all tester strains, in absence and presence of S9 metabolic activation)

Experiment 2:
TA 1535, TA 98, TA 100, TA 102 (in absence and presence of S9 metabolic activation): 100 µg a.s./plate;
TA1537: 50 and 100 µg a.s./plate in absence of S9 metabolic activation; 50 µg a.s./plate in presence of S9 metabolic activation

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

The test substance does not induce reverse mutation in Salmonella typhimurium under the reported experimental conditions. The information contained within this robust summary document comes from studies which are in the ownership of Arch Chemicals Inc. and which are protected in several regions globally. This information may not be used for any purpose other than in support of the Chemical safety Report submitted by Arch Chemicals Inc. under Regulation EC 1907/2006.