2,4,6-tri-n-propyl-2,4,6-trioxo-1,3,5,2,4,6-trioxatriphosphorinane

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-07-04 till 1995-07-06

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Adopted: April 4, 1984

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

July 31, 1992

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Remarks:

Not possible, because the composition of the test water disturbs the analysis by paramagnetic effects

Vehicle:

no

Details on test solutions:

No auxiliary agent was used.
The substance was diluted in water, homogenized with the ultra-turrax and given into test vessels. Afterwards the dilution was stirred for 60 min and the pH was adjusted to a pH of 8 with 10 M NaOH.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Clone: Clone 5 (IRChA, France)
- Source: Stock culture of the Department of Toxicology (Hoechst AG)
- Age of parental stock: up to 42 days
- Type and amount of food during breeding: monocellular green algae (Scenedesmus subspicatus)
- Feeding frequency: 3 times a week
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

20.1 - 20.8

pH:

7.9 - 8.2

Dissolved oxygen:

8.8 - 9.1 mg/L

Nominal and measured concentrations:

Nominal concentrations: 0 and 100 mg/L
no measured concentrations

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL beakers
- Material, size, headspace, fill volume: height 110 mm, diameter 70 mm, water level about 75 mm, fill volume 200 mL
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 10/200 mL

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: Artificial fresh water (according to Elendt)
The dilution water was allowed to stand to stabilize for at least a further 24 hours and was aerated until saturated with oxygen.
- Culture medium different from test medium: no
- Intervals of water quality measurement: once a week

OTHER TEST CONDITIONS
- Adjustment of pH: yes, with 10 m NaOH.
- Photoperiod:12 hours

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Observations on body length and weight: no
- Mortality of control: no
- Abnormal responses: no
- Any observations that might cause a difference between measured and nominal values: no

Validity criteria fulfilled:

yes

Conclusions:

The test item was examined for acute toxicity to aquatic invertetrates in a 48-h static test according to OECD Guideline 202 and EU method C.2. The test item caused no mortality up to a nominal concentration of 100 mg/L. Thus, the 48-h LC50 was determined to be greater than 100 mg/L

Executive summary:

The acute toxicity of the test item to aquatic invertebrates (Daphnia magna) was determined according to the OECD Guideline 202 and EU method C.2.

A static test was performed as a limit test with a concentration of 100 mg/L. Twenty test organisms were exposed to the test concentration and the control, respectively. Mortality and mobility were determined after 24 and 48 hours. The test substance undergoes fast hydrolysis in water with subsequent temperature and pH-dependent changes in the composition of the hydrolysis products. An analytical determination of the test compound or of the hydrolysis products in the test batches was not possible, because the composition of the test water disturbs the analysis by paramagnetic effects.

Test organisms exposed to 100 mg/L of the test item showed no changes in mobility. The test item caused no mortality up to a nominal concentration of 100 mg/L. Thus, the 96-h LC50 was determined to be greater than 100 mg/L.

Description of key information

The test item was examined for acute toxicity to aquatic invertetrates in a 48-h static test according to OECD Guideline 202 and EU method C.2. The test item caused no mortality up to a nominal concentration of 100 mg/L. Thus, the 48-h LC50 was determined to be greater than 100 mg/L

Additional information

The acute toxicity of the test item to aquatic invertebrates (Daphnia magna) was determined according to the OECD Guideline 202 and EU method C.2.

A static test was performed as a limit test with a concentration of 100 mg/L. Twenty test organisms were exposed to the test concentration and the control, respectively. Mortality and mobility were determined after 24 and 48 hours. The test substance undergoes fast hydrolysis in water with subsequent temperature and pH-dependent changes in the composition of the hydrolysis products. An analytical determination of the test compound or of the hydrolysis products in the test batches was not possible, because the composition of the test water disturbs the analysis by paramagnetic effects.

Test organisms exposed to 100 mg/L of the test item showed no changes in mobility. The test item caused no mortality up to a nominal concentration of 100 mg/L. Thus, the 96-h LC50 was determined to be greater than 100 mg/L.