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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
September 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report Date:
2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Specific details on test material used for the study:
Batch: VE00470062

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
In the range-finding test, the test item was carefully applied in the stirring vessel at a loading rate of 100 mg/L without mixing the test item into the water column. Then, the stirring vessel was tightly sealed with glass stoppers. A small headspace had to be included for practical reasons as the test item was floating on the water surface.

Test solutions

Vehicle:
no
Details on test solutions:
The test design included three replicates per test concentration and six replicates for the control.
The main test was performed in a closed system to avoid losses of the test item by evaporation.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
The test organism used for the study was Pseudokirchneriella subcapitata (formerly Selenastrum capricornutum, occasionally also listed as Raphidocelis subcapitata), Strain No. 61.81 SAG, supplied by the Collection of Algal Cultures (SAG, Institute for Plant Physiology, University of Göttingen, 37073 Göttingen / Germany). The algae were cultivated at IES Ltd Laboratories under standardized conditions according to the test guidelines.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Hardness:
The calculated water hardness of the test water was 0.15 mmol/L (= 15 mg/L as CaCO3).
Test temperature:
The test flasks were incubated in a temperature controlled orbital shaker (Multitron-Pro, Infors HT, Bottmingen/Switzerland) at a temperature of 24 °C.
pH:
The pH of the test media was in the range of 7.4 to 7.8 during the test period.
Nominal and measured concentrations:
Based on the results of the range-finding test and in agreement with the Sponsor the following concentrations of NEOFOLIONE were selected for the main test: the equilibrated test medium with a loading rate of 100 mg/l and the dilutions 1:2.2, 1:4.6, 1:10, 1:22 and 1:46. Additionally, a control (test water without test item) was tested in parallel.
The test was started using a nominal algal cell density of 5000 cells/mL. The initial cell density was selected according to the recommendations of the OECD test guideline.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.21 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
0.19 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.16 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

The 72hr EC50 (growth rate) of Neofolione was determined to be 0.21 mg/L.

The 72hr EC10 (growth rate) of Neofolione was determined to be 0.19 mg/L.

The 72hr NOEC (growth rate) of Neofolione was determined to be 0.16 mg/L.