Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
August - September 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report Date:
2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Specific details on test material used for the study:
Batch: VE00470062

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
As the test item is a liquid with low water solubility (<100 mg/L) the slow-stirring method was applied for preparation of an equilibrated test item solution.

Test solutions

Vehicle:
no
Details on test solutions:
For determination of the appropriate test concentrations for the main test, a static range-finding test in a closed system was performed. The test concentrations were the undiluted equilibrated test medium with a loading rate of 100 mg/L and the dilutions 1:10 and 1:100.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
The study was performed with young daphnids of the species Daphnia magna Straus. A clone of this species (originally from the Daphnia Collection of the University of Basel/Switzerland in 2015) is successfully bred in IES Ltd Laboratories.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
250mg/L as CaCO3
Test temperature:
The test was performed in a temperature-controlled room with continuous monitoring of the room temperature. The water temperature was maintained at 21 °C.
Nominal and measured concentrations:
Based on the results of the stirring experiment and the range-finding test , the following concentrations of NEOFOLIONE were selected for the main test: the equilibrated test medium with a loading rate of 100 mg/L and the dilutions 1:2.2, 1:4.6, 1:10, 1:22 and 1:46. Additionally, a control (test water without test item) was tested in parallel.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.4 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.44 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

The 48h EC50 of Neofolione was determined to be 2.4 mg/L.