Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
March 1991
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report Date:
1991

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Specific details on test material used for the study:
Beauversate (alternative trade name for Neofolione): Quest Code 556712, Test Substance Code R647

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
The inoculum used was 10% by volume of activated sludge plant secondary effluent, filtered through a Whatman filter paper (541) to remove coarse particulate matter.
Duration of test (contact time):
28 d
Details on study design:
The test used was the sealed vessel test which is suitable for determining the ready and ultimate biodegradability of organic substances. The test is conducted in 160ml vessels containing 100ml mineral salts medium inoculated with secondary effluent and the respective test or reference substance.

Results and discussion

% Degradation
Key result
Parameter:
% degradation (O2 consumption)
Value:
85.7
Sampling time:
28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Executive summary:

Neofolione undergoes 85.7% biodegradation after 28 days in the test conditions.

Therefore, Neofolione should be regarded as readily biodegradable according to this test.