Esterification product of Nonanoic acid with C9-11, C10-rich alcohols, branched

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GPL guideline study without restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

1

Reference substance name:

Esterification product of Nonanoic acid with C9-11, C10-rich alcohols, branched

EC Number:

946-239-2

Cas Number:

related to 109-32-0

Molecular formula:

C19H38O2 (main), C20H40O2, C18H36O2

IUPAC Name:

Esterification product of Nonanoic acid with C9-11, C10-rich alcohols, branched

Sampling and analysis

Analytical monitoring:

no

Remarks:

A sufficiently sensitive method for analyses in the required concentration range was not available. However all reasonable efforts were taken to produce a saturated solution of all soluble components of the test substance in test media.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test substance is a mixture of sparingly water soluble
components. Therefore test solutions were prepared separately
for each test concentration as a water accommodated fraction
(WAF) using a liquid-liquid saturator technique [Ref. 7, 9]. For each
test solution, a glass aspirator bottle (2-L) with a bottom side-outlet
attached to a stopcock is filled with 2-L test media. Then 231-μL
(considering density = 0.8640 g/cm³) of the test substance was
pipetted carefully on the water surface. The bottle was closed and
the solution was stirred on a magnetic plate for about 2 days. The
remaining undissolved test substance remained on the surface
and the required volume of the WAF (approx. 1-L per test solution)
was removed from the bottom port. Undissolved test substance
was visible at the surface of the test aspirator bottle (oily drops).
The control was treated in the same way.
Fresh test solutions were prepared daily. The test vessels were
conditioned with the test solution for 24-h prior to initiating
exposure.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: The clone of Daphnia magna STRAUS 1820
used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of BASF SE in Ludwigshafen.
- Age at study initiation (mean and range, SD): < 24 hours (at least 3rd brood progeny)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers (nominal volume 100 mL)
- Material, size, headspace, fill volume: 50 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 light:dark
- Light intensity:

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes