Tin bis(2-ethylhexanoate)

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

fish early-life stage toxicity

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to section 13_Analogue Approach Rationale

Reason / purpose for cross-reference:

read-across source

Duration:

120 h

Dose descriptor:

LOEC

Effect conc.:

50 µmol/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

larval development

Remarks on result:

other: source substance

Duration:

120 h

Dose descriptor:

NOEC

Effect conc.:

25 µmol/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

larval development

Remarks on result:

other: source substance

The results of the current study show that the tested compound induced early life stage toxicity in zebrafish embryos and larvae when embryos were exposed from 6 to 120 h. It was revealed that exposure to low doses of SnCl2 (10, 25 µM) did not significantly affect the embryos’ survival or cause obvious malformation during the embryonic stages (6–120 hpf). However, exposure to higher concentrations of SnCl2 (50, 100, and 250 µM) reduced survival and caused changes in embryonic development, including tail deformation, reduced body growth, smaller head and eyes, bent trunk, mild pericardial edema, and smaller caudal fin. These data enriched our knowledge of the influence of SnCl2 on embryonic development. These results were in good agreement with those reported in previous studies related to zebrafish and other fish species.

Validity criteria fulfilled:

yes

Conclusions:

Results clearly indicate a significant dose-dependent increase of developmental and genetic toxicity due to exposure to SnCl2. The NOEC was
determined to be 25 µM/L SnCl_2 (=4,7 mg SnCl_2 / L), higher concentrations than 50µM/L (9,4 mg SnCl_2/ L) cause malformations in embryonic state. If referred to the Sn content in the target substance, the NOEC is calculated as 10,1 mg/L.

Executive summary:

A study with SnCl2 has been conducted accoring to OECD Guideline 212 at concentrations of 10, 25, 50,100 and 250 µM in a static freshwater design. Validity criteria were fulfilled. The results of the current study show that the tested compound induced early life stage toxicity in zebrafish embryos and larvae when embryos were exposed from 6 to 120 h. It was revealed that exposure to low doses of SnCl2 (10, 25 µM) did not significantly affect the embryos’ survival or cause obvious malformation during the embryonic stages (6–120 hpf). However, exposure to higher concentrations of SnCl2 (50, 100, and 250 µM) reduced survival and caused changes in embryonic development, including tail deformation, reduced body growth, smaller head and eyes, bent trunk, mild pericardial edema, and smaller caudal fin. These data enriched our knowledge of the influence of SnCl2 on embryonic development. These results were in good agreement with those reported in previous studies related to zebrafish and other fish species.
Results clearly indicate a significant dose-dependent increase of developmental and genetic toxicity due to exposure to SnCl2. The NOEC was determined to be 25 µM/L SnCl_2 (=4,7 mg SnCl_2 / L), higher concentrations than 50µM/L (9,4 mg SnCl_2/ L) cause malformations in embryonic state. If referred to the Sn content in the target substance, the NOEC is calculated as 10,1 mg/L.

Description of key information

No long-term toxicity to fish study is available for tin bis(2-ethylhexanoate).

An OECD 212 Guideline study with Tin dichloride has been identified and the results will be used for risk characterisation.
Read-across with source SnCl2, OECD 212, static, Danio rerio, NOEC is calculated as 10,1 mg/L.

Further investigations are not necessary according to exposure considerations in Chemical Safety Assessment and environmental behaviour of test material in water.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

NOEC

Effect concentration:

10.1 mg/L