Reaction products of diphenyl ether and 9-methylene nonadecane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Reaction products of diphenyl ether and 9-methylene nonadecane

EC Number:

943-303-1

Molecular formula:

Variable, substance is a UVCB

IUPAC Name:

Reaction products of diphenyl ether and 9-methylene nonadecane

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L (nominal)
- Sampling method: Two sets were collected (fresh and old medium at sample preparation, renewal and the end of exposure). Samples were taken form the middle layer of the relevant vessels.

Test solutions

Vehicle:

no

Details on test solutions:

Test solutions were prepared as Water Accommodated Fractions (WAFs) and Water Soluble Fractions (WSFs). Initially amounts of test substance were weighed and added directly to aspirators where they were stirred for 48 hours. The resulting WAF was then filtered using a 0.45 µm membrane filter. The resulting solutions were then used on study. This procedure was confirmed as a result of the range finding phase, where the physical presence of the test material caused immobility in Daphnids.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: In house cultures
- Feeding during test: No

ACCLIMATION
- Acclimation period: 28 days or less
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Chlorella vulgaris 6 mgC (organic carbon) /2 L/day
- Feeding frequency: As above
- Health during acclimation (any mortality observed): None observed

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Juveniles less than 24 hours old were used for the test.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

No details

Test temperature:

Within the range 20.1 and 20.2°C

pH:

Within the range 7.5 and 7.8.

Dissolved oxygen:

Within the range 7.9 and 8.6 D.O mg/L

Nominal and measured concentrations:

Nominal loading rate: 100 mg/L
Mean measured (TWA): 0.00970 mg/L (0.00838 to 0.0112 mg/L)

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL vessel
- Type: open
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Elendt M4 media as per OECD guideline

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hour light/ 8 hour dark
- Light intensity: 800 Lux or less

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobility (lack of movement within 15 seconds of gentle agitation) assessed at 24 and 48 hours.

RANGE-FINDING STUDY
- Test concentrations: 0, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: Yes WAF and WSF used on main study at a nominal loading rate of 100 mg/L

Reference substance (positive control):

yes

Remarks:

Potassium dichromate test conducted every 6 months

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.01 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: exceeds limit of aqueous solubility

Details on results:

No immobility was observed at a limit concentration of 100 mg/L (loading rate). This mean test concentration at this loading rate was 0.00970 mg/L. The test solutions used on study were colourless with no precipitate evident and the effect concentrations exceeded the solubility of the test substance.

Results with reference substance (positive control):

- Results with reference substance valid: Yes (within historical laboratory range)

Reported statistics and error estimates:

Not applicable as a limit test

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

validity criteria on test fulfilled

Conclusions:

The effect concentrations to Daphnia magna exceeded the aqueous solubility of the test substance when performing a limit test at a loading rate of 100 mg/L. The water solubility of the test substance in the aqueous media was determined to be very low.