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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The 48h-EC50 of 6,6'-di-tert-butyl-4,4'-diethyl-2,2'-methylenediphenol at the water saturation level under the conditions of this test is higher than the limit concentration tested (saturated solution at a loading rate of 100 mg test item/L).

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Additional information

The acute toxicity of6,6'-di-tert-butyl-4,4'-diethyl-2,2'-methylenediphenolto water fleas (Daphnia magna) was tested following the OECD testing Guideline 202 (2004) and according to the GLP Guidelines (Egeler, 2017). Twenty daphnids per group (5 per replicate, quadruplicate) were exposed to an untreated control and to asaturatedsolution at a loading rate of 100 mg test item/L.The total exposure period was 48 hours and samples for analytical confirmation of exposure concentrations were taken at the start and at the end of the test. The limit of detection (LOD) was defined as 30 % of the LOQ (i.e. 0.03 µg/L). All measured test item concentrations were below the LOD. The study met the acceptability criteria prescribed by the study plan and was considered valid. In this limit test no immobilities were observed in control or in the undiluted filtrate of a saturated solution at a loading rate of 100 mg test item/L. It is therefore concluded that the 48h-EC50 of the test itemat the water saturation level under the conditions of this test is higher than the limit concentration tested (saturatedsolution at a loading rate of 100 mg test item/L).