3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarbonyl chloride

Skin sensitisation

Administrative data

Endpoint:

skin sensitisation: in vivo (non-LLNA)

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from safety assessment report

Data source

Reference

Reference Type:

secondary source

Title:

SIDS Dossier

Author:

OECD SIDS

Year:

2008

Bibliographic source:

OECD HPV Chemical programme,SIDS Dossier, approved at SIAM 27 (14-17 October 2008)

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)

Principles of method if other than guideline:

Murine local lymph node assay was conducted on groups of mice at various concentrations on the dorsum of both ears for test chemical Nonanoyl Chloride (CAS no: 764-85-2) to determine its sensitization potential.

GLP compliance:

not specified

Type of study:

mouse local lymph node assay (LLNA)

Justification for non-LLNA method:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Nonanoyl Chloride

Cas Number:

764-85-2

Molecular formula:

C9H17ClO

IUPAC Name:

Nonanoyl Chloride

Test material form:

liquid

Details on test material:

- Name of test material (as cited in study report): Nonanoyl chloride
- Molecular formula: C9H17ClO
- Molecular weight: 176.685 g/mol
- Substance type: organic
- Physical state: liquid
- Smiles: O=C(CCCCCCCC)Cl
- InChI: 1S/C9H17ClO/c1-2-3-4-5-6-7-8-9(10)11/h2-8H2,1H3

Specific details on test material used for the study:

- Name of test material (as cited in study report): Nonanoyl chloride
- Molecular formula: C9H17ClO
- Molecular weight: 176.685 g/mol
- Substance type: organic
- Physical state: liquid
- Smiles: O=C(CCCCCCCC)Cl
- InChI: 1S/C9H17ClO/c1-2-3-4-5-6-7-8-9(10)11/h2-8H2,1H3

In vivo test system

Test animals

Species:

mouse

Strain:

not specified

Sex:

not specified

Details on test animals and environmental conditions:

No data available

Study design: in vivo (LLNA)

Vehicle:

acetone/olive oil (4:1 v/v)

Concentration:

Various concentration (5%, 10% and 15%)

No. of animals per dose:

3

Details on study design:

Nonanoyl chloride in acetone /olive oil was applied to groups of mice for 3 consecutive days in various concentrations on the dorsum of both ears. On day 5 after the first application mice are injected intravenously with [³H]thymidine. Subsequently [³H]thymidine activity was measured as a function of isotope incorporation in the draining atricular lymph nodes.

Positive control substance(s):

not specified

Statistics:

Chemicals, which provoke at least in one concentration, a 3-fold or greater increase in isotope incorporation compared with the vehicle controls are classified as potential sensitizers.

Results and discussion

In vivo (LLNA)

Results

Results 1

Parameter:

other: T/C ratio

Value:

12.7

Test group / Remarks:

at 5% concentration

Remarks on result:

other: sensitizing

Results 2

Parameter:

other: T/C ratio

Value:

19.4

Test group / Remarks:

at 10% concentration

Remarks on result:

other: sensitizing

Results 3

Parameter:

other: T/C ratio

Value:

20.9

Test group / Remarks:

at 25% concentration

Remarks on result:

other: sensitizing

Cellular proliferation data / Observations:

Nonanoyl chloride revealed sensitizing properties in this test.

Any other information on results incl. tables

% Concentration	5	10	25
T/C ratio	12.7	19.4	20.9

Applicant's summary and conclusion

Interpretation of results:

other: sensitizing

Conclusions:

The test material Nonanoyl Chloride (CAS no: 764-85-2) was considered to be sensitizing in the Murine local lymph node assay performed according to OECD 429 Guideline.

Executive summary:

Murine local lymph node assay was conducted on groups of mice at various concentrations on the dorsum of both ears for test chemical Nonanoyl Chloride (CAS no: 764-85-2) to determine its sensitization potential in accordance with OECD 429 Guideline.

 

Nonanoyl chloride in acetone /olive oil was applied to groups of mice for 3 consecutive days in various concentrations on the dorsum of both ears. On day 5 after the first application mice are injected intravenously with [³H]thymidine. Subsequently [³H]thymidine activity was measured as a function of isotope incorporation in the draining atricular lymph nodes. Chemicals, which provoke at least in one concentration, a 3-fold or greater increase in isotope incorporation compared with the vehicle controls are classified as potential sensitizers.

 

Since the chemicalNonanoyl chloride (CAS no: 764-85-2) revealed sensitizing properties in this test, it was considered to be sensitizing in the Murine local lymph node assay.