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Ecotoxicological information

Toxicity to terrestrial arthropods

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Administrative data

Endpoint:
toxicity to terrestrial arthropods: long-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 232 (Collembolan Reproduction Test in Soil)
Deviations:
no
Qualifier:
according to
Guideline:
ISO 11267 (Inhibition of Reproduction of Collembola by Soil Pollutants)
Deviations:
no
GLP compliance:
yes
Application method:
soil

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Details on test material:
- Purity: 98.7%

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes
Remarks:
acetone

Test organisms

Test organisms (species):
Folsomia candida
Animal group:
Collembola (soil-dwelling springtail)

Study design

Study type:
laboratory study
Limit test:
no
Total exposure duration:
28 d

Test conditions

Test temperature:
19 to 21°C
pH (if soil or dung study):
At test start: 6.3 to 6.4
At test end: 6.3 to 6.5
Photoperiod and lighting:
16 h light : 8 h dark
Nominal and measured concentrations:
Nominal: 6.25, 12.5, 25.0, 50.0 and 100 mg/kg soil dry weight, adjusted for purity
Reference substance (positive control):
yes
Remarks:
boric acid

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
> 100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Highest dose tested
Key result
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
> 100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: Highest dose tested
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality and reproduction
Remarks on result:
other: Highest dose tested

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
28 d EC50 and LOEC (Folsomia candida): >100 mg/kg soil dw (Highest concentration tested)
28 d NOEC (Folsomia candida): 100 mg/kg soil dw (Highest concentration tested)
Executive summary:

The effects of the test substance on survival and reproduction of Collembola, Folsomia candida, was measured in a 28-day soil exposure GLP-compliant laboratory study based on OECD 232 and ISO 11267. Eight replicates for the control and four replicates per test item group, containing ten Collembola each (total 80 per control and 40 individuals per test substance group) were each exposed for 28 days to the nominal concentrations of 6.33, 12.66, 25.33, 50.66 and 101.3 mg test substance/kg soil dry weight (corresponding to 6.25, 12.5, 25.0, 50.0 and 100 mg test substance/kg soil dry weight, adjusted for purity) and to an untreated control (using the same amount of acetone and fine quartz sand per g substrate as in the test item groups). The concentrations of test substance adjusted for purity will be used to determine the endpoints of the test. After 28 days exposure the Collembola were assessed for the number of surviving adults (mortality) and the number of juveniles (reproduction).

The reference item boric acid is tested once a year. In the most recent test performed, the EC50 for reproduction was determined to be 107.2 mg boric acid/kg soil dry weight.

No significant effects were observed up to and including the concentration of 100 mg/kg soil dry weight.

The test substance had no significant lethal or reproductive effects on the Collembola Folsomia candida when exposed to concentrations up to and including 100 mg/kg artificial soil dry weight.

The 28 day EC50 and the Lowest-Observed-Effect Concentration (LOEC) for the test substance were determined to be greater than 100 mg/kg artificial soil dry weight. The overall 28 day No-Observed-Effect Concentration (NOEC) based on mortality and reproduction was determined to be 100 mg/kg artificial soil dry weight.