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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: EEC Guidelines for the Testing of Chemicals, The Artificial Soil Test, EEC 79/831, Rev. 3
Deviations:
no
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Details on test material:
- Purity: 96.8%

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes
Remarks:
acetone

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d

Test conditions

Test temperature:
20 ± 2°C
pH:
6 ± 0.5
Moisture:
artificial soil had 30% moisture content
Nominal and measured concentrations:
0.1, 1, 10, 100, and 1000 mg/kg (a.s./soil dry weight)
Reference substance (positive control):
yes
Remarks:
Chloroacetamide

Results and discussion

Effect concentrations
Key result
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other: Highest dose tested

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
14 d LC50 (E. fetida): >1000 mg/kg soil dw (mortality) (highest concentration tested)
Executive summary:

The objective of this study was to evaluate the toxicity of the test substance to the earthworm (Eisenia fetida), confined to treated artificial soil for fourteen days. The methods described in this study are consistent with EEC 79/831, Rev. 3. The test substance was tested at concentrations of 0.1, 1, 10, 100, and 1000 mg a.s./kg soil dry weight. The standard, chloroacetamide, was used at concentrations of 2, 4, 8, 16, 32, and 64 mg/kg. Earthworm mortality was determined at 7 and 14 days post treatment.

Soil applications of the test substance had no effect on the earthworm. There was no mortality in any of the test units treated with the test substance. Hence, the LC50 was estimated to be greater than 1000 mg a.s./kg soil dry weight, the highest rate tested. Also, weight loss during the course of the study was no greater than that sustained in the control units. LC50 for the standard, chloroacetamide, was 22.6 mg/kg. There was no mortality in the control units.