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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From March 9, 1983 to April 2, 1983
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report Date:
2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: OECD Guideline 406
Principles of method if other than guideline:
As part of a Magnusson-Kligman guinea pig maximization, shaved clilpped shoulders of male guinea pigs were patched with 25% citronellyl-3-methyl-2-butenoate in vaseline for 48 hours under occlusion. Citronellyl-3-methyl-2-butenoate induced slight skin irritation in 5/10 guinea pigs.

In a primary irritation study, Pirbright white guinea pigs weighing 300-500 g were topically treated on the shorn flank daily with 50 % test material olive oil for 5 days. The test solution was rubbed in for 30 seconds at each application. Animals were individually housed and provided feed and water ad libitum. The room temperature was 20 °C with a humidity of 50 % and a dark/light cycle of 12 hours.
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Type:
Constituent
Test material form:
liquid

Test animals

Species:
guinea pig
Strain:
not specified
Details on test animals and environmental conditions:
TEST ANIMALS
- Sex: Male
- Weight at study initiation: 200 - 400 g

Test system

Type of coverage:
occlusive
Preparation of test site:
shaved
Vehicle:
other: vaseline
Controls:
not specified
Amount / concentration applied:
TEST MATERIAL
- Concentration (if solution): 25 %
Duration of treatment / exposure:
The test solution was applied during 48 hours.
Number of animals:
10 animals

Results and discussion

In vivo

Results
Irritation parameter:
erythema score
Time point:
48 h
Remarks on result:
probability of weak irritation
Irritant / corrosive response data:
The topical applications with the 25% dilution of the test substance in vaseline, made in the induction phase, induced erythema in five out of ten animals.

Applicant's summary and conclusion

Interpretation of results:
not irritating
Conclusions:
As part of a Magnusson-Kligman guinea pig maximization, shaved clilpped shoulders of male guinea pigs were patched with 25% citronellyl-3-methyl-2-butenoate in vaseline for 48 hours under occlusion.
Citronellyl-3-methyl-2-butenoate induced slight skin irritation in 5/10 guinea pigs.
Not sufficient for classification.