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EC number: 205-448-2 | CAS number: 141-02-6
The toxicity of the test item Dioctylfumarate to zebra fish (Danio rerio) was investigated in an early-life stage toxicity test according to the OECD Guideline for Testing of Chemicals, No. 210, "Fish, Early-life Stage Toxicity Test", 1992.
Freshly fertilized eggs of zebra fish were exposed to test media containing the test item at nominal concentrations of 0.015, 0.047, 0.15, 0.47, and 1.5 mg/L under flow-through conditions for the test period of 35 days. Additionally, a control and a solvent control were tested in parallel.
The preparation of the test media was based on the OECD series on testing and assessment No. 23 on Aquatic Toxicity Testing of Difficult Substances and Mixtures, 2000.
At the start of the test, 60 eggs each (divided into 4 replicates) were distributed to the test concentrations and control and solvent control. The eggs, larvae and juvenile fish were observed for toxic effects on their development, growth and survival.
The nominal test concentrations of 0.47 and 1.5 mg/L were determined in this test as the overall NOEC and LOEC (NOEC and LOEC of the most sensitive test parameter), and therefore, were analytically verified.
The test item concentrations in the analyzed test medium of nominal 0.47 mg/L varied in the range from 54 to 91% of the nominal value. In the test medium of nominal 1.5 mg/L the range was 15 to 143% of the nominal value. The reason for the high variation of the measured test concentrations at the highest test concentration of nominal 1.5 mg/L is considered to be the water solubility of the test item in the test water. The water solubility was determined in a pre-experiment to be approximately 1.1 mg/L. Therefore, slightly above the solubility limit of the test item in test water was tested to result with the mean measured concentration as close as possible at the water solubility limit of 1.1 mg/L.
The mean measured test item concentrations (calculated as the arithmetic mean of all measurements of each test concentration) were 0.30 mg/L and 0.86 mg/L, corresponding to 65 and 58% of the nominal values of 0.47 mg/L and 1.5 mg/L, respectively.
The biological results are related to the mean measured test item concentrations.
Mean measured concentration (mg/L)
Development rate of eggs:
Survival of larvae and juvenile fish:
Fish wet weight and dry weight:
Summarizing the NOEC values for each of the test parameters assessed, the overall NOEC of dioctylfumarate for early life stages of zebra fish was determined to be the mean measured test concentration of 0.30 mg/L, since no toxic effect on the eggs, larvae or fish was observed up to and including this concentration. The overall LOEC was determined to be the mean measured test concentration of 0.86 mg/L, due to the statistically significantly reduced hatching rate at that concentration.
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