Registration Dossier
Registration Dossier
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EC number: 280-960-7 | CAS number: 83817-60-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: screening test, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 May - 22 Jun 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- 2008
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, Klimaschutz, Landwirtschaft und Verbraucherschutz, Germany
Test material
- Reference substance name:
- Ethyl 9-oxo-9H-thioxanthene-2-carboxylate
- EC Number:
- 280-960-7
- EC Name:
- Ethyl 9-oxo-9H-thioxanthene-2-carboxylate
- Cas Number:
- 83817-60-1
- Molecular formula:
- C16H12O3S
- IUPAC Name:
- ethyl 9-oxo-9H-thioxanthene-2-carboxylate
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: sewage treatment plant Rossdorf, Germany
- Preparation of inoculum for exposure: aliquots of washed sludge suspension, corresponding to 3.5 g dry material/L were mixed with test water and aerated overnight
- Pretreatment: sludge was washed by centrifugation, liquid phase was then decanted and solid material was resuspended in tap water and centrifuged, procedure was repeated 3 times. An aliquot of the final sludge suspension was weighed, dried and ratio of wet sludge to dry weight was determined. - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 103.3 mg/L
- Based on:
- test mat.
- Initial conc.:
- 220.8 mg/L
- Based on:
- other: ThODNH4
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Solubilising agent: none used
- Test temperature: 22 °C ± 1 °C
- pH: 7.4 - 8.0
- Suspended solids concentration: 28.7 mg sludge/L per flask
- Continuous darkness: yes
- Other: continuous stirring
TEST SYSTEM
- Culturing apparatus: Manometric Test System with test flasks containing a volume of approx. 500 mL
- Number of culture flasks/concentration: 2
- Measuring equipment: BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany
- Test performed in closed vessels: yes
- Details of trap for CO2: potassium hydroxide solution (45%)
SAMPLING
- Sampling method: by measuring the pressure decrease in the reaction vessels
CONTROL AND BLANK SYSTEM
- Inoculum control: 2 flasks
- Abiotic sterile control: 1 flask (poisoned with HgCl2)
- Toxicity control: 1 flask
- Other: procedure control: 1 flask
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 102.0 mg/L corresponding to an oxygen demand of about 170.0 mg/L (ThODNH4)
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- ThODNH4
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Toxicity control: 36% biodegradation after 14 d and 42% after 28 d (the test item can be assumed to be not inhibitory to the aerobic activated sludge microorganisms as degradation was >25% within 14 d.)
BOD5 / COD results
- Results with reference substance:
- 88% biodegradation of reference substance after 14 d.
Any other information on results incl. tables
Oxygen demand of the inoculum control: 35 mg O2/L
pH of test item flasks after 28 d: 7.4
Reference item degradation: > 60% after 6 d
Biodegradation in toxicity control: 36% after 14 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
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