Hexane, 1,6-diisocyanato-, homopolymer, 2-hydroxyethyl acrylate- and propylene glycol monoacrylate-blocked

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From December 12, 2016 to December 16, 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

DOC determination using a TOC analyser

Details on sampling:

At the start and at the end of the test, the content of the test substance in the test solutions was determined using DOC determination.

Vehicle:

no

Details on test solutions:

The water-accommodated fractions (WAF) were prepared for the test. This was done by weighing the respective nominal loads of the test substance, adding the corresponding amount of tap water and shaking vigorously for 23 h. The resulting solutions were filtered through 0.45 μm membrane filters. The real load of the test substance in mg/L were 4.6; 10.0; 22.0; 46.1; 100.1.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Specification: Species Danio rerio HAMILTON-BUCHANAN, Age: sexually immature young fish, length: 2 +/- 1 cm, Origin: The animals were obtained from a commercial supplier. Before being used for the test, the fish were kept for 12 d under test conditions. During this period, mortality did not surpass 5%. Supplier: Dehner Garten Center, 67433 Neustadt, Date of arrival: 19. October 2016.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

not specified

Test temperature:

20.9 – 23.0°C

pH:

7.8 - 8.1

Dissolved oxygen:

8.3 - 9.7 mg/L

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

Nominal (mg/L): 4.6; 10.0; 22.0; 46.1; 100.1
Measured in t = 0 h and t = 96 h (mg/L) geometric mean: 2.3; 4.5; 6.3; 16.7; 33.3
Nominal and measured concentrations of the test substance in blank controls: 0

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

7.9 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the 96 h measured LC50 of the substance was determind to be 7.9 mg/L (equivalent to nominal level of 25 mg/L) (Muckle, 2017).

Executive summary:

A study was conducted to determine the toxicity of the substance to Danio rerio according to OECD Guideline 203 and EU Method C.1, in compliance with GLP. Water-accommodated fractions (WAF) were prepared for the test. This was done by weighing the respective nominal loads of the test substance, adding the corresponding amount of tap water and shaking vigorously for 23 h. The resulting solutions were filtered through 0.45 μm membrane filters. The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L nominal concentration. At the start and at the end of the test, the content of the test substance in the test solutions was determined using DOC determination. The determined test substance concentrations based on measurement of DOC ranged between 28 and 55% of the nominal concentration. The total exposure period was 96 h. The three top concentrations showed mortality between 14 and 100%. Under the study conditions, the 96 h measured LC50 of the substance was determined to be 7.9 mg/L (equivalent to a nominal level of 25 mg/L) (Muckle, 2017).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

7.9 mg/L

Additional information

A study was conducted to determine the toxicity of the substance to Danio rerio according to OECD Guideline 203 and EU Method C.1, in compliance with GLP. Water-accommodated fractions (WAF) were prepared for the test. This was done by weighing the respective nominal loads of the test substance, adding the corresponding amount of tap water and shaking vigorously for 23 h. The resulting solutions were filtered through 0.45 μm membrane filters. The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L nominal concentration. At the start and at the end of the test, the content of the test substance in the test solutions was determined using DOC determination. The determined test substance concentrations based on measurement of DOC ranged between 28 and 55% of the nominal concentration. The total exposure period was 96 h. The three top concentrations showed mortality between 14 and 100%. Under the study conditions, the 96 h measured LC50 of the substance was determined to be 7.9 mg/L (equivalent to nominal level of 25 mg/L) (Muckle, 2017).