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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 - 27 Jun 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reference:
Composition 0
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
1992
Deviations:
no
GLP compliance:
yes
Test material information:
Composition 1
Analytical monitoring:
yes
Remarks:
TOC determination
Details on sampling:
- Concentrations: all test item concentrations and the control
- Sampling method: at test start and test end
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test item was directly weighed into test water and treated for 60 sec at 8000 rpm with an ultra-turrax and stirred afterwards for 24 h with an a magnetic stirrer. Undissolved particies were removed via a folded filter of the pore size 7-12 µm.
- Controls: yes, test medium control
- Eluate: no
- Differential loading: yes
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Age of test animals: 0 - 24 h
- Keeping conditions: according to internal SOP

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Separation of test animals according to size before start of test: 1st sieve: 14.06.1999 at 2.00 p.m. and 2nd sieve: 15.06.1999 at 8:00 a.m.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
15.5 °dH (M4 medium)
Test temperature:
19.4 °C (at test end, control and test item concentration)
pH:
8.1 (at test end, control)
8.0 (at test end, test item concentration)
Dissolved oxygen:
8.6 mg/L (at test start and test end, control and test item concentration)
Nominal and measured concentrations:
nominal: control, 100 mg/L
Details on test conditions:
TEST SYSTEM
Test vessel:
- Type: open
- Material, size, headspace, fill volume: cylindrical test vessels, diameter 4.0 cm, height 6.5 cm, 20 mL test medium
- Aeration: not ventilated
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-Medium according to Elendt and BGA (1992)

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark

EFFECT PARAMETERS MEASURED: Immobilisation was measured after 24 and 48 h.

VEHICLE CONTROL PERFORMED: no (not required)

RANGE-FINDING STUDY: no range-finding test was performed
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 97.3 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: arithmetic mean of analytical values
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 97.3 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: arithmetic mean of analytical values
Details on results:
- Behavioural abnormalities: no
- Mortality of control: no

Analytical Results:

All values refer to TOC determinations. According to the relevant product informations 1 mg/L TOC equals to 1.4 mg/L of the test substance (structural formula: C10-H14-02; molecular weight: 166.24 g/mole).

The test result refers to a concentration of the test substance, which was calculated directly from analytically determined TOC values. According to the relevant product informations, 1 mg/L equals to 1.4 mg/L of the test substance structural formula; molecular weight: 166.24 g/mole).

Nominal concentration test substance

Actual concentrations (based on arithmetic mean measured concentrations)

TOC results by start of test and after 48 hours [mg/L]

[mg/L]

[mg/L]

0 hours

48 hours

Control

0

< 2

3

100

97.3

71

68

Validity criteria fulfilled:
yes
Conclusions:
The test item had no acute toxic effects on Daphnia magna in a 48-h static test according to EU Method C.2 and GLP up to 100 mg/L (nominal). Immobilisation of Daphnia magna was observed after 48 h resulting in an EC50 > 100 mg/L (> 97.3 mg/L based on the arithmetic mean).

Description of key information

EC50 (48 h) >100 mg/L (EU C.2, Daphnia magna, nominal)

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

One experimental study is available investigating the short-term effects of the substance to aquatic invertebrates. The study was performed according to the EU Method C.2 (GLP) with the water flea Daphnia magna. A limit test with a nominal concentration of 100 mg/L including a control was performed under static conditions. Test concentrations were verified by TOC-analysis at the beginning and end of the exposure period. The nominal concentration of the highest test item concentration was 71 mg/L at test start (corresponds to 99.4 mg/L based on arithemtic mean measured concentration) and 68 mg/L at test end (corresponds to 95.2 mg/L based on arithemtic mean measured concentration). Immobilisation of D.magna was not observed after 48 h resulting in an EC50 (48 h) of >100 mg/L (>97.3 mg/L based on the arithmetic mean measured concentration).