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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
OECD 201
Reference:
Composition 0
Qualifier:
according to
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Deviations:
no
Qualifier:
according to
Guideline:
other: The Notice on the Test Method Concerning New Chemical Substances
Deviations:
no
GLP compliance:
yes
Test material information:
Composition 1
Analytical monitoring:
yes
Vehicle:
not specified
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by dissolving the substance in test medium.
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
72 h
Test temperature:
21.8 - 22ºC
Nominal and measured concentrations:
100, 32, 10, 3.2, 1.0 mg/L (geometric ratio; square root of 10), control (100, 32, 9.3, 2.8, 0.79 mg/L as measured concentration)
Details on test conditions:
TEST SYSTEM
- Material, size, headspace, fill volume: 600 mL/control (100 mL/vesel), 300 mL/concentration (100 mL/vessel)
- No. of vessels per concentration (replicates):3
- No. of vessels per control (replicates):6

OTHER TEST CONDITIONS
- Light intensity and quality:92 - 94 microE/m2/s

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Measurement of biomass: cell concentration
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
ca. 2.8 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
not specified
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
ca. 19 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
not specified
Basis for effect:
growth rate
Remarks on result:
other: 95 % CL: impossible to calculate
Validity criteria fulfilled:
not specified
Conclusions:
Test item has value of EC50 (72 h) of 19 mg/L and NOEC (72 h) of 2.8 mg/L based on growth rate in 72 hours, testing to Algae.
Executive summary:

Test item was tested for the toxicity to Algae in 72 hours test, in the nominal concentration: 100, 32, 10, 3.2, 1.0 mg/L, control and (100, 32, 9.3, 2.8, 0.79 mg/L as measured concentration) at temperature of 21.8 - 22ºC. Three vessels replicates per concentration and 6 vessels replicates per control were used in this study. Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by dissolving the substance in test medium. Based on available data from National instititute of technology and evaluation (NITE), test item has Effective concentration EC50 (72h) of 19 mg/L and NOEC (72h) of 2.8mg/L based on growth rate.

Description of key information

Key study: Based on available data from National instititute of technology and evaluation (NITE) test item has Effective concentration EC50 (72h) of 19 mg/L and NOEC (72h) of 2.8mg/L based on growth rate.

Key value for chemical safety assessment

EC50/LC50 for freshwater algae:
19 mg/L
EC10, LC10 or NOEC for freshwater algae:
2.8 mg/L

Additional information

Key study:OECD TG 201 and GLP principles followed.

Test item was tested for the toxicity to Algae (Pseudokirchnerella subcapitata) in the nominal concentration: 100, 32, 10, 3.2, 1.0 mg/L, control and (100, 32, 9.3, 2.8, 0.79 mg/L as measured concentration) at temperature of 21.8 - 22ºC during 72 hours.Test solutions were prepared by mixing a stock solution of the test substance, which was prepared by dissolving the substance in test medium. Based on available data from National instititute of technology and evaluation (NITE), test item has Effective concentration EC50 (72h) of 19 mg/L and NOEC (72h) of 2.8mg/L based on growth rate.