Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study and GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014
Report Date:
2014

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
CAS No. 82428-30-6
Appearance slight yellow liquid
Water solubility 3.35 g/L (20°C)
Molecular weight 196.25 g/mol
Formula C11H16O3
Density 1.079 g/mL at 20°C
Recommended storage Cool, protected from light

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
62.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In this study S-100; 3,4-Epoxycyclohexylmethyl methacrylate was found to inhibit the growth of the freshwater green alga Desmodesmus subspicatus after 72 hours with the following effect values: The NOEC-values for both inhibition of growth rate and yield after 72 hours were for 25.0 mg/L. The LOEC-values for both inhibition of growth rate and yield after 72 hours were 50. mg/L. The EC50-value for inhibition of growth rate (ErC50) after 72 hours was 62.3 (55.1 – 82.4) mg/L.
The EC50-value for inhibition of yield (EyC50) with 95 % confidence intervals after 72 hours was 48.5 (40.3 – 52.4) mg/L.
All effect levels are given based on nominal test item concentrations of S-100; 3,4-Epoxycyclohexylmethyl methacrylate.
Executive summary:

The toxicity of S-100; 3,4-Epoxycyclohexylmethylmethacrylate (batch number:12051006) to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201 (2011) and Council Regulation (EC) No. 440/2008/ Method C.3 at Dr.U.Noack-Laboratorienin 31157 Sarstedt, Germany from 2014 -10 -21 to 2014 -10 -28 with the definitive exposure phase from 2014 -10 -21 to 2014 -10 -24.The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours.

 

The study was conducted under static conditions with an initial cell density of 4998 cells/mL. Based on a preliminary test, 5 concentration levels were tested in a geometrical series with a dilution factor of 2 (nominal): 6.25 - 12.5 - 25.0 - 50.0 - 100mg/L.Three replicates were tested for each test item concentration and six replicates for the control.Environmental conditionswere determined to be within the acceptable limits.

 

All test concentrations of S-100; 3,4-Epoxycyclohexylmethylmethacrylateand the control were analytically verified by HPLC-DAD analysis at the start of the exposure (0 hours) and the end of the exposure (72 hours).
The measured concentrations of S-100; 3,4 -Epoxycyclohexylmethylmethacrylateat the start of the exposure were in the range of 96 % to 102 % of the nominal values. At the end of the exposure, they were in the range of 90 to 100 %.
All effect values given are based on the nominal test item concentrations of S-100; 3,4-Epoxycyclohexylmethylmethacrylate.