Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12 August 2014 - 10 September 2014
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study and GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
23 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
99
Sampling time:
28 d
Remarks on result:
other: Parallel 1
Details on results:
Biodegradation [%] of the Test Item S-100, 3,4-Epoxycyclohexylmethyl methacrylate in Comparison to the Functional Control and Toxicity Control
Biodegradation [%]
Study Day [d]
Replicate 7 14 21 28
Test Item 23 mg/L
1 25 60 90 99
2 25 49 79 90
Functional Control
45 mg/L 86 97 100 100
Toxicity Control
23 mg/L Test Item +
45 mg/L Reference Item 62 82 88 92

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Both test item replicates reached the 10 % level (beginning of biodegradation) on day 5. At test end the biodegradation of the 1st replicate reached 99 % and of the 2nd replicate 90 %. The mean biodegradation after 28 days was 95%. The 60 % pass level was reached by the mean of replicates on day 16. The 10-d-window was missed marginally by the mean of replicates, but the first replicate complied to the 10-d-window (day 5 to day 14). As a consequence, the test item is considered to be readily biodegradable.
Executive summary:

Both test item replicates reached the 10 % level (beginning of biodegradation) on day 5. At test end the biodegradation of the 1streplicate reached 99 % and of the 2ndreplicate 90 %. The mean biodegradation after 28 days was 95%. The 60 % pass level was reached by the mean of replicates on day 16. The 10-d-window was missed marginally by the mean of replicates, but the first replicate complied to the 10-d-window (day 5 to day 14). As a consequence, the test item is considered to be readily biodegradable.