Tetrachloro-p-benzoquinone

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well performed OECD and GLP guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Version / remarks:

of 1981

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Tetrachloro-p-benzoquinone

EC Number:

204-274-4

EC Name:

Tetrachloro-p-benzoquinone

Cas Number:

118-75-2

Molecular formula:

C6Cl4O2

IUPAC Name:

2,3,5,6-tetrachloro-1,4-benzoquinone

Details on test material:

- Name of test material (as cited in study report): Chloranil

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

New Zealand White rabbits conventional breeding, with appropriate range of bodyweight (2.0 to 2.7 kg).
Room temperature: 20°+- 3°C
Humidity (%): 50+-20.
Commercially available rabbit diet and hay were fed. Deionized, chlorinated water was fed ad libitum.

Test system

Type of coverage:

semiocclusive

Preparation of test site:

shaved

Vehicle:

other: polyethylene glycol 400

Controls:

not required

Amount / concentration applied:

0.5 g test substance mixed with 0.2 ml polyethylene glycol 400.

Duration of treatment / exposure:

4 hours (remnant test substance was removed by rinsing with lukewarm water)

Observation period:

Examinations at 30-60 min, 24, 48, and 72h after patch removal.

Number of animals:

3

Details on study design:

Only animals with intact skin were used. Control animals were not required, as untreated skin areas of the test animal can serve as control. After the 4-hour exposure period residual test substance was cautiously removed from the exposed skin. The treated skin patches were evaluated at: 0.5-1 hour post end of exposure and at 24, 48 and 72 h post end of exposure.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

other: animal # 1, 2, 3 each

Time point:

other: mean of 24, 48, 72 h

Score:

0

Reversibility:

other: initial, slight response in a single animal was fully reversible within 24h

Results 2

Irritation parameter:

edema score

Basis:

other: animal 1, 2, 3 each

Time point:

other: mean of 24, 48,72 h

Score:

0

Reversibility:

other: no signs of irritation at any time

Other effects:

No other effects were reported.

Any other information on results incl. tables

Gradings of Skin Reactions Attained for each Animal at Different Observation Time Points
Time after Patch Removal	0.5 – 1 h	24 h	48 h	72 h
Animal (Identification) No.	1  2  3	1  2  3	1  2  3	1  2  3
Erythema	0  1  0	0  0  0	0  0  0	0  0  0
Edema	0  0  0	0  0  0	0  0  0	0  0  0

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

The mean Draize scores for erythema and edema derived from 24, 48 and 72 hour post treatment observation time points in 3 rabbits were 0.0 each. The only erythema finding (very slight in degree) seen in this study in one animal at 0.5 to 1 hour post patch removal was completely reversible within 24 hours post patch removal. Based on these finding the test substance is neither skin irritating nor skin corrosing.

Executive summary:

2,3,5,6-tetrachloro-1,4-benzoquinone was tested for skin irritation according to OECD Guideline 404 and in compliance with GLP. Reliability grade 1 was assigned to the study. Each of three New Zealand White rabbits was treated for 4 hours by semi-occlusive administration of a single dose of 0.5 g test substance moistened with polyethylene glycol 400 to shaved intact skin (approximately 6 cm2/animal). The site of administration was examined for skin reactions at 0.5-1, 24, 48 and 72 hours post patch removal. The only finding during this study was an erythema very slight in degree (Grade 1, barely perceptible) in one animal at 0.5 -1 hour post patch removal. This finding was completely reversible within 24 hours post patch removal. No edema reaction was evident during the entire study. Mean Draize scores for erythema and edema derived from the latter three observation time points were 0.0 each. Based on these finding the test substance is neither skin irritating nor skin corrosing.