2-chlorophenothiazine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02/03/2016 - 30/03/2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-chlorophenothiazine

EC Number:

202-152-5

EC Name:

2-chlorophenothiazine

Cas Number:

92-39-7

Molecular formula:

C12H8ClNS

IUPAC Name:

2-chloro-10H-phenothiazine

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: provide by the sponsor, Lot n. 150009
- Expiration date of the lot/batch:
- Purity test date: 99.1%

RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity:
- Specific activity:
- Locations of the label:
- Expiration date of radiochemical substance:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: for 10 days at 4 ± 4°C until storage recommendations were provided; thereafter at 20 ± 5°C in the dark
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

FORM AS APPLIED IN THE TEST (if different from that of starting material)

OTHER SPECIFICS:SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: provided by the sponsor, Lot n.150009
- Expiration date of the lot/batch:
- Purity test date: 99.1% (HPLC)

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: After receipt at the test facility for 10 days at 4 ± 4°C until storage recommendations were provided; thereafter at 20 ± 5°C in the dark
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

FORM AS APPLIED IN THE TEST (if different from that of starting material)

OTHER SPECIFICS:

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

28 d

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (test mat. analysis)

Remarks:

THOD NH4

Value:

3

Sampling time:

28 d

% Degradation 2

Parameter:

% degradation (test mat. analysis)

Remarks:

ThOD NO3

Value:

2

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The mean biodegradation at test end of the test after 28 days was 3% and 2%, respectively (THODNH4, ThODNO3).
Therefore, 2-CHLOROPHENOTHIAZINE is considered to be not readily biodegradable.