trans-menthone

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

calculation (if not (Q)SAR)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

accepted calculation method

Justification for type of information:

Data is from computational model developed by USEPA

Qualifier:

according to guideline

Guideline:

other: Modeling database

Principles of method if other than guideline:

Estimation of 96 hr EC50 value of test chemical by modelling Database - ECOSAR v.1.11. The ECOSAR Class Program is a computerized version of the ECOSAR analysis procedures as currently practiced by the Office of Pollution Prevention and Toxics (OPPT).  It has been developed within the regulatory constraints of the Toxic Substances Control Act (TSCA).  It is a pragmatic approach to SAR. The structure-activity relationships (SARs) presented in this program are used to predict the aquatic toxicity of chemicals based on their similarity of structure to chemicals for which the aquatic toxicity has been previously measured.  Most SAR calculations in the ECOSAR Class Program are based upon the octanol/water partition coefficient (Kow).  Various surfactant SAR calculations are based upon the average length of carbon chains or the number of ethoxylate units.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one- Common name: Menthone - Molecular formula: C10H18O- Molecular weight: 154.2512 g/mol- Smiles notation: C1([C@@H](CC[C@@H](C1)C)C(C)C)=O- InChl: 1S/C10H18O/c1-7(2)9-5-4-8(3)6-10(9)11/h7-9H,4-6H2,1-3H3- Substance type: Organic- Physical state: Liquid

Analytical monitoring:

not required

Details on sampling:

No data available

Vehicle:

not specified

Details on test solutions:

No data available

Test organisms (species):

other: green algae

Details on test organisms:

No data available

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Remarks on exposure duration:

Estimated data

Post exposure observation period:

No data available

Hardness:

No data available

Test temperature:

No data available

pH:

No data available

Dissolved oxygen:

No data available

Salinity:

No data available

Conductivity:

No data available

Nominal and measured concentrations:

No data available

Details on test conditions:

No data available

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

13.399 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Neutral Organics (ECOSAR)

Details on results:

No data available

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

No data available

Validity criteria fulfilled:

not specified

Conclusions:

The estimated EC50 values for aquatic green algae after 96 hours was 13.399 mg/l on the basis of growth rate.

Executive summary:

Based on the prediction done using theEPI Suite ECOSAR version 1.10, the short term toxicity on green algae was predicted for test substance menthone (89 -80 -5). On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 13.399 mg/l for green algae for 96 hrs duration. Based on this value, it can be concluded that the test chemical menthone can be considered as toxic to green algae at environmentally relevant concentrations and can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

Description of key information

Based on the prediction done using theEPI Suite ECOSAR version 1.10, the short term toxicity on green algae was predicted for test substance menthone (89 -80 -5). On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 13.399 mg/l for green algae for 96 hrs duration. Based on this value, it can be concluded that the test chemical menthone can be considered as toxic to green algae at environmentally relevant concentrations and can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:

13.399 mg/L

Additional information

Based on the various predicted and experimental data for the target chemical study have been reviewed to determine toxic nature of menthone (89-80-5) (2R, 5S)-5-methyl-2-(propan-2-yl) cyclohexan-1-one on the growth and other activity of algae. The studies are as mentioned below:

 

In the first weight of evidence study for target chemical menthone toxicity was measured according to the ECOSAR. Based on the prediction done using the EPI Suite ECOSAR version 1.10, the short term toxicity on green algae was predicted for test substance menthone (89 -80 -5). On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 13.399 mg/l for green algae for 96 hrs duration. Based on this value, it can be concluded that the test chemical menthone can be considered as toxic to green algae at environmentally relevant concentrations and can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

 

First study was supported by another study which was conducted for read across chemical from secondary sources. Study of toxicity of test chemical on the growth of green algae for 72 hrs was carried out. Test performed in accordance with OECD Guideline 201 (Alga, Growth Inhibition Test) under static system. After the exposure of chemical with the green algae the EC50 and NOEC value was measured. Based on the growth rate inhibition of algae the EC50 and NOEC was >2.5 and 2.5mg/l. But on the basis of AUG the EC5O and the NOEC was >70 mg/l. Thus based on the EC50 (>70 mg/l) value by considering AUG, chemical was concluded as toxic and can be consider to be classified as chronic 3 category.

 

Similar results was obtain for another read across chemical from secondary source. Short term toxicity study of test chemical on the growth of Scenedesmus subspicatus (Algae) was carried out for 72 hours. The study was performed according the standard OECD guideline 201. Test conducted under the static system. Control and test material is used in the test. Based on the growth inhibition of algae due to the test chemical for 72 hrs. The NOEC was 5 mg/l, LOEC was 10mg/l and the ErC50 was 16.2 mg/l. Based on the EC50, chemical was consider as toxic and can be consider to be classified in aquatic chronic category 3 as per the CLP classification criteria.

 

Based on the data from various databases and secondary sources for target menthone (89-80-5) and read across chemicals, menthone was toxic and can be consider as classified as aquatic chronic 3 category as per mention in CLP classification criteria.