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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
calculation (if not (Q)SAR)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
accepted calculation method
Justification for type of information:
Data is from computational model developed by USEPA
Reference:
Composition 0
Qualifier:
according to
Guideline:
other: Modeling database
Principles of method if other than guideline:
Estimation of 96 hr EC50 value of test chemical by modelling Database - ECOSAR v.1.11. The ECOSAR Class Program is a computerized version of the ECOSAR analysis procedures as currently practiced by the Office of Pollution Prevention and Toxics (OPPT).  It has been developed within the regulatory constraints of the Toxic Substances Control Act (TSCA).  It is a pragmatic approach to SAR. The structure-activity relationships (SARs) presented in this program are used to predict the aquatic toxicity of chemicals based on their similarity of structure to chemicals for which the aquatic toxicity has been previously measured.  Most SAR calculations in the ECOSAR Class Program are based upon the octanol/water partition coefficient (Kow).  Various surfactant SAR calculations are based upon the average length of carbon chains or the number of ethoxylate units.
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
- Name of test material (IUPAC name): (2R,5S)-5-methyl-2-(propan-2-yl)cyclohexan-1-one- Common name: Menthone - Molecular formula: C10H18O- Molecular weight: 154.2512 g/mol- Smiles notation: C1([C@@H](CC[C@@H](C1)C)C(C)C)=O- InChl: 1S/C10H18O/c1-7(2)9-5-4-8(3)6-10(9)11/h7-9H,4-6H2,1-3H3- Substance type: Organic- Physical state: Liquid
Analytical monitoring:
not required
Details on sampling:
No data available
Vehicle:
not specified
Details on test solutions:
No data available
Test organisms (species):
other: green algae
Details on test organisms:
No data available
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Remarks on exposure duration:
Estimated data
Post exposure observation period:
No data available
Hardness:
No data available
Test temperature:
No data available
pH:
No data available
Dissolved oxygen:
No data available
Salinity:
No data available
Conductivity:
No data available
Nominal and measured concentrations:
No data available
Details on test conditions:
No data available
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
13.399 mg/L
Nominal / measured:
estimated
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: Neutral Organics (ECOSAR)
Details on results:
No data available
Results with reference substance (positive control):
No data available
Reported statistics and error estimates:
No data available
Validity criteria fulfilled:
not specified
Conclusions:
The estimated EC50 values for aquatic green algae after 96 hours was 13.399 mg/l on the basis of growth rate.
Executive summary:

Based on the prediction done using theEPI Suite ECOSAR version 1.10, the short term toxicity on green algae was predicted for test substance menthone (89 -80 -5). On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 13.399 mg/l for green algae for 96 hrs duration. Based on this value, it can be concluded that the test chemical menthone can be considered as toxic to green algae at environmentally relevant concentrations and can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

Description of key information

Based on the prediction done using theEPI Suite ECOSAR version 1.10, the short term toxicity on green algae was predicted for test substance menthone (89 -80 -5). On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 13.399 mg/l for green algae for 96 hrs duration. Based on this value, it can be concluded that the test chemical menthone can be considered as toxic to green algae at environmentally relevant concentrations and can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

Key value for chemical safety assessment

EC50/LC50 for freshwater algae:
13.399 mg/L

Additional information

Based on the various predicted and experimental data for the target chemical study have been reviewed to determine toxic nature of menthone (89-80-5) (2R, 5S)-5-methyl-2-(propan-2-yl) cyclohexan-1-one on the growth and other activity of algae. The studies are as mentioned below:

 

In the first weight of evidence study for target chemical menthone toxicity was measured according to the ECOSAR. Based on the prediction done using the EPI Suite ECOSAR version 1.10, the short term toxicity on green algae was predicted for test substance menthone (89 -80 -5). On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance is estimated to be 13.399 mg/l for green algae for 96 hrs duration. Based on this value, it can be concluded that the test chemical menthone can be considered as toxic to green algae at environmentally relevant concentrations and can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

 

First study was supported by another study which was conducted for read across chemical from secondary sources. Study of toxicity of test chemical on the growth of green algae for 72 hrs was carried out. Test performed in accordance with OECD Guideline 201 (Alga, Growth Inhibition Test) under static system. After the exposure of chemical with the green algae the EC50 and NOEC value was measured. Based on the growth rate inhibition of algae the EC50 and NOEC was >2.5 and 2.5mg/l. But on the basis of AUG the EC5O and the NOEC was >70 mg/l. Thus based on the EC50 (>70 mg/l) value by considering AUG, chemical was concluded as toxic and can be consider to be classified as chronic 3 category.

 

Similar results was obtain for another read across chemical from secondary source. Short term toxicity study of test chemical on the growth of Scenedesmus subspicatus (Algae) was carried out for 72 hours. The study was performed according the standard OECD guideline 201. Test conducted under the static system. Control and test material is used in the test. Based on the growth inhibition of algae due to the test chemical for 72 hrs. The NOEC was 5 mg/l, LOEC was 10mg/l and the ErC50 was 16.2 mg/l. Based on the EC50, chemical was consider as toxic and can be consider to be classified in aquatic chronic category 3 as per the CLP classification criteria.

 

Based on the data from various databases and secondary sources for target menthone (89-80-5) and read across chemicals, menthone was toxic and can be consider as classified as aquatic chronic 3 category as per mention in CLP classification criteria.