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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Qualifier:
according to
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
Commercial Name: Baccartol Crude
Givaudan Code No.: 2376504
Aspect: yellow liquid
Lot No.: VE00410612
Purity: Conform
Expiration Date: January 31, 2018
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois-de-Bay, Satigny, Switzerland) was used.

The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day
Duration of test (contact time):
62 d
Initial conc.:
30 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Test performance:
Oxygen uptakes, as read on the Oxitop meters, are corrected to account for the small differences between actual and nominal concentrations of test and reference substances.
Degradation of sodium benzoate exceeded 40 % after 7 days and 65 % after 14 days: the activity of the inoculum was thus verified (validity criterion).
Parameter:
% degradation (O2 consumption)
Value:
51
Sampling time:
28 d
Parameter:
% degradation (O2 consumption)
Value:
62
Sampling time:
56 d
Parameter:
% degradation (O2 consumption)
Value:
63
Sampling time:
62 d
Details on results:
At the test concentration, Baccartol Crude did not inhibit the intrinsic respiration of the inoculum.
Validity criteria fulfilled:
yes
Interpretation of results:
not inherently biodegradable
Conclusions:
The Inherent Biodegradability of Baccartol Crude was determined by the Manometric Respirometry Test according to the OECD Guidelines for Testing of Chemicals, Method No. 302C.

In the test conditions Baccartol Crude undergoes 51% biodegradation after 28 days (62% biodegradation after 56 days, 63% biodegradation after 62 days).

At the test concentration, Baccartol Crude did not inhibit the intrinsic respiration of the inoculum.

According to this test, Baccartol Crude cannot be regarded as inherently and ultimately biodegradable.
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Qualifier:
according to
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
Qualifier:
according to
Guideline:
EPA OPPTS 835.3110 (Ready Biodegradability)
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
Commercial Name: Baccartol Crude
Givaudan Code No.: 2376504
Aspect: yellow liquid
Lot No.: VE00410612
Purity: Conform
Expiration Date: January 31, 2018
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois-de-Bay, Satigny, Switzerland) was used.

The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.
Duration of test (contact time):
66 d
Initial conc.:
30 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Test performance:
Oxygen uptakes, as read on the Oxitop controller, are corrected to account for the small differences between actual and nominal concentrations of test and reference substances.
Degradation of sodium benzoate exceeded 40% after 7 days and 65% after 14 days: the activity of the inoculum was thus verified (validity criterion).
The repeatability validity criterion (not more than 20% difference between replicates) is fulfilled. Therefore, the test is considered valid.
Parameter:
% degradation (O2 consumption)
Value:
46
Sampling time:
28 d
Details on results:
At the test concentration Baccartol Crude did inhibit the intrinsic respiration of the inoculum by more than 20% on day 1 of the test. Toxicity towards the inoculum at test concentration can therefore not be excluded.

Baccartol Crude should be regarded as not readily biodegradable according to this test.
Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The Ready Biodegradability of Baccartol Crude was determined by the Manometric Respirometry Test.

Baccartol Crude undergoes 46% biodegradation after 28 days in the test conditions.

At the test concentration Baccartol Crude did inhibit the intrinsic respiration of the inoculum by more than 20% on day 1 of the test. Toxicity towards the inoculum at test concentration can therefore not be excluded.

Baccartol Crude should be regarded as not readily biodegradable according to this test.

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