4-acetamido-5-[[4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]anthranilic acid, sodium salt

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrate

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic invertebrate predicted for 2-amino-4-acetamido-5-[(E)-2-(4-{[2-(sulfooxy)ethane]sulfonyl}phenyl)diazen-1-yl]benzoic acid sodium (CAS: 94158-82-4). Effect concentration i.e EC50 value estimated to be 675.47 mg/l for Daphnia magna for 48 hrs duration. It can be concluded that the 2-amino-4-acetamido-5-[(E)-2-(4-{[2-(sulfooxy)ethane]sulfonyl}phenyl)diazen-1-yl]benzoic acid sodium (CAS: 94158-82-4) is likely to be not toxic to aquatic inveretebreate, hence it can be considered to be“not classified”as per the CLP classification criteria for aquatic environment.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic algae predicted for 2-amino-4-acetamido-5-[(E)-2-(4-{[2-(sulfooxy)ethane]sulfonyl}phenyl)diazen-1-yl]benzoic acid sodium (CAS: 94158 -82-4). Effect concentration i.e EC50 value estimated to be 400.69 mg/l for Desmodesmus subspicatus for 72 hrs duration. It can be concluded that the 2-amino-4-acetamido-5-[(E)-2-(4-{[2-(sulfooxy)ethane]sulfonyl}phenyl)diazen-1-yl]benzoic acid sodium (CAS: 94158-82-4) is likely to be not toxic to aquatic algae, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

Additional information

Short-term toxicity to aquatic invertebrate

Following studies include the predicted data and experimental study for the target chemical and structurally similar read across to conclude the toxicity extent of 2-amino-4-acetamido-5-[(E)-2-(4-{[2-(sulfooxy)ethane]sulfonyl}phenyl)diazen-1-yl]benzoic acid sodium (CAS: 94158-82-4) towards aquatic invertebrate is summarized as follows:

 

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic invertebrate predicted for 2-amino-4-acetamido-5-[(E)-2-(4-{[2-(sulfooxy)ethane]sulfonyl}phenyl)diazen-1-yl]benzoic acid sodium (CAS: 94158-82-4). Effect concentration i.e EC50 value estimated to be 675.47 mg/l for Daphnia magna for 48 hrs duration. It can be concluded that the 2-amino-4-acetamido-5-[(E)-2-(4-{[2-(sulfooxy)ethane]sulfonyl}phenyl)diazen-1-yl]benzoic acid sodium (CAS: 94158-82-4) is likely to be not toxic to aquatic inveretebreate, hence it can be considered to be“not classified”as per the CLP classification criteria for aquatic environment.

 

The above predicted data of target chemical is supported by the experimental study of structurally similar read across disodium 5-acetamido-4-hydroxy-3-(phenyldiazenyl)naphthalene-2,7-disulfonate (CAS: 3734-67-6) from the ABITEC study report, suggests that An acute immobilisation test was used to test how a range of concentrations of CAS 3734-67-6 exerts different degrees of toxic effects on the swimming capability of Daphnia magna under otherwise identical test conditions. The test was performed in close resemblance to OECD guideline 202 by ABITEC in Prague. The standardized protocol used to obtain the herein described results was by the testing laboratory referred to as“CSN EN ISO 6341”. The testing aim was to determine a EC50 after 48 hours of exposure to D. magna. Daphnids were exposed to CAS 3734-67-6 in 50 ml beakers in a volume of 25 ml of liquid solution containing both the chemical and media as specified in OECD 202. The beakers were placed in a temperature controlled room at 20±1 degrees Celsius. TheD. magna(age≤24) used for the test had been breed at ABITEC. The breeding stock ofD. magnaoriginated from University of Technology in Prague. The animals were exposed to medium (i.e.a beaker containing only medium) and/or the tested chemical during 48 hours (±1 hour). None of the exposed animal’s immobilization were affected by exposure to only medium. The nominal concentrations used were: 100 mg/L (limit test). There were 5 Daphnia per test vessels and 4 replicates per concentration. The pH in test vessels were 7.7-7.8 mg/L. The positive control/reference substance used in the tested showed an expected result and gave an EC50 that corresponded to previous exposures with this chemical in D. magna. The EC50 was defined as a concentration that immobilizes 50% of the exposed D. magna. Eight percent of immobilization in D. magna after 48 hours of exposure to 100 mg/L of CAS 3734-67-6 was observed. The EC8 was therefore estimated to be 100 mg/L.

Hence from the value it can be considered as "not classifed" as per classfication criteria for aquatic environment.

 

Similar experimental study of another structurally similar read across N,N-dimethylformamide (CAS: 68-12-2) from publication Archives of Environmental Contamination and Toxicology 1983, indicates that the

Short-term toxicity to aquatic invertebrates test was carried tout to study the effect of N,N-dimethylformamide at 21 C , pH 7.9 - 8.3, total hardness: 165 mg/l for 48 hrs. Mortality effect was measured. The lethal concentration LC50 for daphnia magna at 48 hrs is 12350 mg/l. It can be concluded that the N,N-dimethylformamide is not toxic to aquatic environment and applying the weight of evidence approach it can be considered as“not classified”as per CLP regulation.

 

Thus based on the effect concentrations which is in the range 100.00 mg/l to 1235 mg/l give the conclusion that test substance 2-amino-4-acetamido-5-[(E)-2-(4-{[2 (sulfooxy)ethane]sulfonyl}phenyl)diazen-1-yl]benzoic acid sodium (CAS: 94158-82-4) was likely to be not toxic to aquatic invertebrate at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be“not classified”as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Following studies include the predicted data and experimental study for the target chemical and structurally similar read across to conclude the toxicity extent of 2-amino-4-acetamido-5-[(E)-2-(4-{[2-(sulfooxy)ethane]sulfonyl}phenyl)diazen-1-yl]benzoic acid sodium (CAS: 94158-82-4) towards aquatic algae is summarized as follows:

 Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic algae predicted for 2-amino-4-acetamido-5-[(E)-2-(4-{[2-(sulfooxy)ethane]sulfonyl}phenyl)diazen-1-yl]benzoic acid sodium (CAS: 94158 -82-4). Effect concentration i.e EC50 value estimated to be 400.69 mg/l for Desmodesmus subspicatus for 72 hrs duration. It can be concluded that the 2-amino-4-acetamido-5-[(E)-2-(4-{[2-(sulfooxy)ethane]sulfonyl}phenyl)diazen-1-yl]benzoic acid sodium (CAS: 94158-82-4) is likely to be not toxic to aquatic algae, hence it can be considered to be“not classified”as per the CLP classification criteria for aquatic environment. 

The above predicted data of target chemical is supported by the experimental study of structurally similar read across disodium 5-acetamido-4-hydroxy-3-(phenyldiazenyl)naphthalene-2,7-disulfonate (CAS: 3734-67-6) from the ABITEC study report, suggests that A freshwater algal growth inhibition exposure assessment was used to test how a range of concentrations of CAS 3734-67-6 exerts different degrees of toxic effects on the growth of Desmodesmus subspicatusunder otherwise identical test conditions. The test was performed in close resemblance to OECD guideline 201 by ABITEC in Prague, Crez Republic. The standardized protocol used to obtain the herein described results was by the testing laboratory referred to as“CSN EN ISO 8692”. The 72 hours EC50 was by the testing laboratory defined as the concentration estimated to inhibit 50 per cent of the algae cell growth compared to unexposed cells after 27 hours of exposure. The nominal concentrations used for the exposure assessment were as follows:0, 20, 30, 45, 67 and 100 mg/L. The positive control/reference substance used in the tested showed an expected result and gave a EC50 that corresponded to previous exposures with this chemical in Desmodesmus subspicatus. In conclusion, the EC50 for CAS 3734-67-6 was 285.8 mg/L (95% C.I.: 173.5-470.8 mg/L) after 72 hours of exposure. It can be concluded from the value that the disodium 5-acetamido-4-hydroxy-3-(phenyldiazenyl)naphthalene-2,7-disulfonate is not toxic to the aquatic algae and can be considered as "not classified" as per the classification criteria for the aquatic environment.

Similar experimental study of another structurally similar read across N,N-dimethylformamide (CAS: 68-12-2) from publication Bulletin of Environmental Contamination and Toxicology 1983, indicates that theTo study the aquatic toxicity of N,N-dimethylformamide the Static bottle test according to Miller, et al.(1978) or EPA-600/9-78-018 was carried out. In the highest concentration (1 ml/l) a statistically significant increase of algal growth was observed with respect to dry weight. The NOEC value for Selenastrum capricornutum in 14 days is 470 mg/l. It can be concluded that the N,N-dimethylformamide is not toxic and applying the weight of evidence approach it can be considered as“not classified”as per CLP regulation.

 

Thus based on the effect concentrations which is in the range 285.8 mg/l to 470 mg/l give the conclusion that test substance 2-amino-4-acetamido-5-[(E)-2-(4-{[2 (sulfooxy)ethane]sulfonyl}phenyl)diazen-1-yl]benzoic acid sodium (CAS: 94158-82-4) was likely to be not toxic to aquatic invertebrate at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be“not classified”as per the CLP classification criteria.