Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions; publication with detailed description of test procedure

Data source

Reference
Reference Type:
publication
Title:
The Effects of Organic Environmental Chemicals on the Growth of the Alga Scenedesmus subspicatus: A Contribution to Environmental Biology
Author:
Geyer H et al.
Year:
1985
Bibliographic source:
Chemosphere, Vol 14, No. 9, pp 1355-1369, 1985

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Version / remarks:
test period was 96 h instead of 72 h
Deviations:
yes
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
(epoxyethyl)benzene
EC Number:
202-476-7
EC Name:
(epoxyethyl)benzene
Cas Number:
96-09-3
Molecular formula:
C8H8O
IUPAC Name:
2-phenyloxirane

Sampling and analysis

Analytical monitoring:
not specified

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISMThe usually unicellular freshwater green alga Scenedesmus subspicatus Chodat (86, 81 SAG), belonging to the order of Chlorococcales (Chlorophyta, Chlorophyceae), was obtained from Dr. Renate KGhn, Institute for Water, Soil and Air Hygiene, Federal Health Office, Berlin-Dahlem, FRG.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Reference substance (positive control):
yes
Remarks:
Pentachlorphenol, Atrazine

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
ca. 32 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
cell number
Duration:
96 h
Dose descriptor:
EC10
Effect conc.:
12 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
cell number
Results with reference substance (positive control):
EC 50 (96h):0.09 mg/l (Pentachlorphenol)0.11 mg/l ((Atrazine)

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The Toxicity of Styrene oxide to Scenedesmus subspicatus is Low
Executive summary:

In a 96 hour acute toxicity study conducted similar to the OECD guideline 201, the cultures of Scenedesmus subspicatus (new name: Desmodesmus subspicatus) were exposed to Styrene oxide under static conditions.  The EbC10and EbC50values based on cell density were 12 and 32 mg a.i./L, respectively. There were no compound related phytotoxic effects.

This toxicity study is classified as reliable with restrictions and satisfies the requirements for algal growth inhibition study.

 

 

Results Synopsis

Test Organism: Scenedesmus subspicatus

Test Type (Static): OECD Guideline 201

96hr EbC10:  12 mg/L

96hr EbC50: 32 mg/L

Endpoint(s) Effected: Biomass