α,α,α-trichlorotoluene

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The authors performed a cell multiplication inhibition test to assess if benzotrichloride is hazardous to the protozoa Entosiphon sulcatum. The methodology is explained with great detail and the study is generally well described. Thus we consider this a Klimisch 2e study as the study is well documented, meets generally accepted scientific principles and is acceptable for assessment.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Bestimmung der biologischen Schadwirkung wassergefährdender Stoffe gegen Protozoen. I. Bakterienfressende Flagellaten (Modellorganismus: Entosiphon sulcatum Stein)

Author:

Bringmann G.

Year:

1978

Bibliographic source:

Z. f. Wasser- und Abwasser-Forschung, 11(6): 210-215

Reference 2

Reference Type:

publication

Title:

Comparison of the toxicity thresholds of water pollutants to bacteria, algae, and protozoa in the cell multiplication inhibition test

Author:

Bringmann G. and Kühn R.

Year:

1980

Bibliographic source:

Water Research, 14: 231-241

Materials and methods

Principles of method if other than guideline:

The authors performed a cell multiplication inhibition test. Using this method the onset of the inhibition of cell multiplication under the influence of the test substance is determined.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

α,α,α-trichlorotoluene

EC Number:

202-634-5

EC Name:

α,α,α-trichlorotoluene

Cas Number:

98-07-7

Molecular formula:

C7H5Cl3

IUPAC Name:

α,α,α-trichlorotoluene

Constituent 2

Reference substance name:

trichloromethylbenzene

IUPAC Name:

trichloromethylbenzene

Details on test material:

- Name of test material (as cited in study report): benzotrichlorid

No more data available

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Entosiphon sulcatum

Study design

Test type:

static

Water media type:

not specified

Limit test:

no

Total exposure duration:

72 h

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

other: toxicity threshold

Effect conc.:

56 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: similar to EC5

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Conclusions:

The authors performed a cell multiplication inhibition test to assess if benzotrichloride is hazardous to the protozoa Entosiphon sulcatum. Under these testing conditions the toxicity threshold (i.e. similar to EC5) for Entosiphon sulcatum after 72h of exposure was 56 mg/L (nominal concentration).

Executive summary:

The authors performed a cell multiplication inhibition test to assess if benzotrichloride (CAS n° 98-07-7) is hazardous to the protozoa Entosiphon sulcatum. Under static conditions, Entosiphon sulcatum was exposed to a dilution series of the test substance at 25°C for 72h. Then growth inhibition was assessed using a cell counter comparing the values of the cultures with test substance to a blanc. Under these testing conditions the toxicity threshold (i.e. similar to EC5) for Entosiphon sulcatum was 56 mg/L (nominal concentration).

No data is available on the GLP status and no guideline is followed. However, the methodology used in this study is described with sufficient details and the study itself meets generally accepted scientific principles and is as such acceptable for assessment. Therefore we consider the study reliable with restrictions (klimisch 2e).