Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from RIFM report, Robust study summaries and authoritive database HSDB and HPVIS
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Principles of method if other than guideline:
Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance (2E)-2-(phenylmethylidene)heptanal. The study was performed according to OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test).
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material: (2E)-2-(phenylmethylidene)heptanal
- Molecular formula: C14H18O
- Molecular weight: 202.2952 g/mol
- Smiles notation: c1(\C=C(\CCCCC)C=O)ccccc1
- InChl: 1S/C14H18O/c1-2-3-5-10-14(12-15)11-13-8-6-4-7-9-13/h4,6-9,11-12H,2-3,5,10H2,1H3/b14-11-
- Substance type: Organic
- Physical state: Liquid
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Concentration of sludge: 30 mg/L
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
other: BOD
Details on study design:
TEST CONDITIONS
- Other: Bottle 1 & 2: Basal culture medium + activated sludge 30 mg/l + test chemical (100 mg/l);
Bottle 3: Basal culture medium + activated sludge 30mg/l+ aniline (100mg/l);
Bottle 4: Basal culture medium + activated sludge 30
Reference substance:
aniline
Key result
Parameter:
other: BOD
Value:
90
Sampling time:
28 d
Remarks on result:
other: readily biodegradable
Details on results:
Test substance undergoes 90% degradation by BOD parameter in 28 days.
Results with reference substance:
Reference substance undergoes 61% degradation in 28 days.
Validity criteria fulfilled:
not specified
Interpretation of results:
readily biodegradable
Conclusions:
The percentage degradation of test substance (2E)-2-(phenylmethylidene)heptanal was determined to be 90% by BOD parameter in 28 days. Thus, based on percentage degradation, (2E)-2-(phenylmethylidene)heptanal is considered to be readily biodegradable in water.
Executive summary:

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance (2E)-2-(phenylmethylidene)heptanal (CAS no. 122-40-7) under aerobic conditions.The study was performed according to OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. Aniline was used as a reference substance for the study. Test vessel involve theBottle 1 & 2: Basal culture medium + activated sludge 30 mg/l + test chemical (100 mg/l); Bottle 3: Basal culture medium + activated sludge 30mg/l+ aniline (100mg/l) and Bottle 4: Basal culture medium + activated sludge 30 mg/l. Reference substance undergoes 61% degradation in 28 days.The percentage degradation of test substance (2E)-2-(phenylmethylidene)heptanalwas determined to be 90% by BOD parameter in 28 days.Thus, based on percentage degradation, (2E)-2 -(phenylmethylidene)heptanal is considered to be readily biodegradable in nature.

Description of key information

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance (2E)-2-(phenylmethylidene)heptanal (CAS no. 122-40-7) under aerobic conditions (A. M. Api et. al, 2015).The study was performed according to OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. Aniline was used as a reference substance for the study. Test vessel involve theBottle 1 & 2: Basal culture medium + activated sludge 30 mg/l + test chemical (100 mg/l); Bottle 3: Basal culture medium + activated sludge 30mg/l+ aniline (100mg/l) and Bottle 4: Basal culture medium + activated sludge 30 mg/l. Reference substance undergoes 61% degradation in 28 days.The percentage degradation of test substance (2E)-2-(phenylmethylidene)heptanalwas determined to be 90% by BOD parameter in 28 days.Thus, based on percentage degradation, (2E)-2 -(phenylmethylidene)heptanal is considered to be readily biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

Various experimental studies for the target compound (2E)-2 -(phenylmethylidene)heptanal (CAS No. 122-40-7) were reviewed for the biodegradation end point which are summarized as below:

 

In an experimental key study from peer reviewed journal (A. M. Api et. al, 2015), biodegradation experiment was conducted for 28 days for evaluating the percentage biodegradability of test substance (2E)-2-(phenylmethylidene)heptanal (CAS no. 122-40-7) under aerobic conditions. The study was performed according to OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. Aniline was used as a reference substance for the study. Test vessel involve the Bottle 1 & 2: Basal culture medium + activated sludge 30 mg/l + test chemical (100 mg/l); Bottle 3: Basal culture medium + activated sludge 30mg/l+ aniline (100mg/l) and Bottle 4: Basal culture medium + activated sludge 30 mg/l. Reference substance undergoes 61% degradation in 28 days. The percentage degradation of test substance (2E)-2-(phenylmethylidene)heptanel was determined to be 90% by BOD parameter in 28 days.Thus, based on percentage degradation, (2E)-2 -(phenylmethylidene)heptanal is considered to be readily biodegradable in nature.

 

In an supporting study from authoritative database (J-CHECK, 2017), biodegradation experiment was conducted for 28 days for evaluating the percentage biodegradability of test substance (2E)-2-(phenylmethylidene)heptanal (CAS no. 122-40-7).The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I)). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of test substance (2E)-2-(phenylmethylidene)heptanal was determined to be 51 and 81% by BOD and TOC removal parameter in 28 days. Thus, based on percentage degradation, (2E)-2 -(phenylmethylidene)heptanal is considered to be readily biodegradable in nature.

 

Another biodegradation study was conducted according to OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test) under aerobic condition for 28 days for evaluating the percentage biodegradability of test substance (2E)-2-(phenylmethylidene)heptanal (CAS no. 122-40-7) (HSDB, 2017). Secondary effluent from an unacclimatized activated sludge was used as a test inoculum. 10-day window criteria has been fulfilled. The percentage degradation of test substance (2E)-2-(phenylmethylidene)heptanal was determined to be 10 and 65% by CO2 evolution parameter after 10 and 28 days, respectively. Thus, based on percentage degradation, (2E)-2-(phenylmethylidene)heptanal is considered to be readily biodegradable in nature.

 

In an supporting study from peer reviewed journal (A.M. Api et. al, 2015),biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance (2E)-2-(phenylmethylidene)heptanal (CAS no. 122-40-7). The study was performed according to OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test). The percentage degradation of test substance (2E)-2 -(phenylmethylidene) heptanal was determined to be 70.5% by CO2 evolution parameter in 28 days. Thus, based on percentage degradation, (2E)-2 -(phenylmethylidene)heptanal is considered to be readily biodegradable in nature.

 

For the target chemical(2E)-2-(phenylmethylidene)heptanal(CAS No. 122-40-7), Biodegradation study was conducted according to Commission Directive 79/831/EWG annex V part C method for 28 days for evaluating the percentage biodegradability of test substance (2E)-2-(phenylmethylidene)heptanal (A.M. Api et. al, 2015). The percentage degradation of test substance (2E)-2-(phenylmethylidene)heptanal was determined to be41% in 28 days. Thus, based on percentage degradation, (2E)-2 -(phenylmethylidene)heptanal is considered to be inherently biodegradable in nature.

 

On the basis of above results for target chemical (2E)-2 -(phenylmethylidene)heptanal (from peer reviewed journal, authoritative database J-CHECK and HSDB, 2017), it can be concluded that the test substance (2E)-2 -(phenylmethylidene)heptanal can be expected to be readily biodegradable in nature.