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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Readily biodegradable (according to OECD criteria).

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

The biodegradation potential of the substance in water was determined in a screening study according to OECD TG 301B (CO2-Evolution Test) and in compliance with GLP criteria (BASF, 2015). In this study ca. 29 mg/L test substance (20 mg/L TOC) was inoculated with activated sludge from domestic sewage for 28 days under aerobic conditions in the dark. During the incubation period degradation was followed by determining the carbon dioxide produced. After the 28-day incubation period 80 - 90 % of the test substance was biodegraded. The pass level of 60 % degradation was reached within 14 days after the start of the test. The 10-window criterion is not applied as the test substance is a mixture of structure similar compounds which are typically characterized by different kinetic degradation steps. Based on these findings, the test substance is classified as readily biodegradable.

The biodegradation potential of the substance in water was further examined under anearobic conditions in a study according to DIN 38414, Part 8 (BASF, 2014). In this study ca. 1.6 g test substance (300 mg/L based on DOC) was inoculated with digested sludge from a sewage treatment plant under anearobic conditions. During the incubation period degradation was followed by determining the formation of methane. After a 48-day incubation period 64 % of the added test substance was degraded. In a repeat experiment under similar conditions, the anaerobic degradation was 93 %.