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EC number: 203-481-7 | CAS number: 107-31-3
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- publication
- Title:
- A Study of Micronucleus Induction with Methyl Formate and 2-Methylbutane in Bone Marrow Cells of Male ICR Mice
- Author:
- Soo-Jin KIM, Kyung-Taek RIM, Min-Gu KANG, Jong-Kyu KIM, Yong-Hyun CHUNG and Jeong-Sun YANG
- Year:
- 2 010
- Bibliographic source:
- Safety and Health at Work
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- not specified
- Type of assay:
- mammalian erythrocyte micronucleus test
Test material
- Reference substance name:
- Methyl formate
- EC Number:
- 203-481-7
- EC Name:
- Methyl formate
- Cas Number:
- 107-31-3
- Molecular formula:
- C₂H₄O₂
- IUPAC Name:
- methyl formate
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- ICR
- Sex:
- male
Administration / exposure
- Route of administration:
- oral: unspecified
- Vehicle:
- Olive oil
- Duration of treatment / exposure:
- 24 hours
Doses / concentrationsopen allclose all
- Dose / conc.:
- 250 mg/kg bw/day (nominal)
- Dose / conc.:
- 500 mg/kg bw/day (nominal)
- Dose / conc.:
- 1 000 mg/kg bw/day (nominal)
- No. of animals per sex per dose:
- 6
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Mitomycin C (0,5 mg/kg bw)
Examinations
- Tissues and cell types examined:
- 2,000 polychromatic erythrocytes of bone marrow (PCEs, reticulocytes; immature erythrocytes) were scored per animal for the frequency of micronucleated cells in each of the 6 animals per dosage group
- Details of tissue and slide preparation:
- Bone marrow cells were obtained from the femurs immediately following sacrifice.
- Evaluation criteria:
- Cells were considered to be micronucleated when they neatly contained defined chromatin corpuscles with a diameter of less than one-third the diameter of the cell nucleus and stained equal or lighter than the nucleus of the cell from which the micronucleated cell was developed with a DNA specific fluorescence dye.
- Statistics:
- The experimental and control micronucleus frequency within and between the different dose groups were compared with the One Way ANOVA test and the Kruskal-Wallis tests (α=0.05).
Results and discussion
Test results
- Key result
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- not specified
- Negative controls validity:
- not specified
- Positive controls validity:
- not specified
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
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