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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions. Restriction: not reported whether treatment-related body weight changes observed.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Deviations:
yes
Remarks:
; body weight was not controlled
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl formate
EC Number:
203-481-7
EC Name:
Methyl formate
Cas Number:
107-31-3
Molecular formula:
C₂H₄O₂
IUPAC Name:
methyl formate
Details on test material:
- Test substance: Methyl formate
- Analytical purity: 9
- Impurity. methanol 2%.

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: males 250 g, females 180 g

Administration / exposure

Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
TEST SITE
- Area of exposure: backside, 42 cm²
- % coverage:

Doses:
2000 and 4000 mg/kg bw
No. of animals per sex per dose:
3
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: animals were observed daily for signs of toxicity. Mortalities were recorded.
- Necropsy of survivors performed: yes/no all animals were examined after death or at termination.
- Other examinations performed: clinical signs, body weight,organ weights, histopathology.

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 4 000 mL/kg bw
Mortality:
No mortality seen at any dose within the 14-d observation period.
Clinical signs:
Slight apathy, stagger, unsteady gait, unregular breathing. No signs of local irritation noted.

Applicant's summary and conclusion

Executive summary:

Conclusion:

There were no deaths (0/12) in rats after dermal contact to methyl formate at the Limit Test concentration and at 4000 mg/kg bw.