Methyl formate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions. Restriction: not reported whether treatment-related body weight changes observed.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

; body weight was not controlled

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl formate

EC Number:

203-481-7

EC Name:

Methyl formate

Cas Number:

107-31-3

Molecular formula:

C₂H₄O₂

IUPAC Name:

methyl formate

Details on test material:

- Test substance: Methyl formate
- Analytical purity: 9
- Impurity. methanol 2%.

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: males 250 g, females 180 g

Administration / exposure

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: backside, 42 cm²
- % coverage:

Doses:

2000 and 4000 mg/kg bw

No. of animals per sex per dose:

3

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: animals were observed daily for signs of toxicity. Mortalities were recorded.
- Necropsy of survivors performed: yes/no all animals were examined after death or at termination.
- Other examinations performed: clinical signs, body weight,organ weights, histopathology.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 4 000 mL/kg bw

Mortality:

No mortality seen at any dose within the 14-d observation period.

Clinical signs:

Slight apathy, stagger, unsteady gait, unregular breathing. No signs of local irritation noted.

Applicant's summary and conclusion

Executive summary:

Conclusion:

There were no deaths (0/12) in rats after dermal contact to methyl formate at the Limit Test concentration and at 4000 mg/kg bw.