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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

OECD 202, GLP, Klimisch 1.  Daphnia acute 48 hour EC50 5.18 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
5.18 mg/L

Additional information

The acute toxicity of the test item OLEIC_DimerFA_TETA_PAA (batch no.: BB 100860 V1) to Daphnia magna was determined according to OECD-Guideline for Testing of Chemicals No. 202 (2004) at Dr.U.Noack-Laboratorien, 31157 Sarstedt, Germany. the study was conducted in natural river water.

A static test was performed with the nominal concentrations of 0.625 - 1.25 - 2.50 - 5.00 - 10.0 mg/L. The test media were prepared in natural river water. Duration of the test was 48 hours. Twenty test organisms were exposed to each concentration level (4 replicates each with test organisms) and a control. Water quality parameters pH-value, temperature and oxygen-saturation, measured after 0, 24 and 48 h, were determined to be within the acceptable limits.


OLEIC_DimerFA_TETA_PAA has low water solubility and may sorb to organic and inorganic materials by different mechanisms. Due to these properties the test item was difficult to test in artificial water (e.g. sorption to the test organism and walls of the test vessel). Natural river water contains particulate as well as dissolved organic carbon to which the test item can sorb partially preventing that the test item settles onto surfaces. The sorbed fraction of the test item was difficult to extract from the test system which normally leads to low analytical recoveries. Nevertheless the test item was present in the test system and therefore available for exposure (dissolved in water and sorbed).


No determinations of the test item concentrations were carried out. Preliminary investigations including the use of different analytical techniques have shown that no specific method was available that met the required sensitivity in the range of the test concentrations.


All effect levels are given based on nominal concentrations of OLEIC_DimerFA_TETA_PAA.

The 48 h EC50 of OLEIC_DimerFA_TETA_PAA to Daphnia magna was 5.18 mg/L (95% confidence limits: 2.64 - 9.86 mg/L) and the corresponding NOEC was 2.50 mg/L.