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Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
data is from peer reviewed publications

Data source

Reference
Reference Type:
publication
Title:
Fragrance material review on benzyl acetate
Author:
D. McGinty , D. Vitale, C.S. Letizia, A.M. Api
Year:
2012
Bibliographic source:
Food and Chemical Toxicology 50 (2012) S363–S384

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
To assess the ocular irritation potential of benzyl acetate in rabbits
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzyl acetate
EC Number:
205-399-7
EC Name:
Benzyl acetate
Cas Number:
140-11-4
Molecular formula:
C9H10O2
IUPAC Name:
benzyl acetate
Test material form:
liquid
Details on test material:
- Name of test material (as cited in study report): Benzyl acetate
- Molecular formula: C9H10O2
- Molecular weight: 150.176 g/mol
- Substance type: organic
- Physical state: Liquid
- Smiles notation: c1(COC(C)=O)ccccc1
- InChl: 1S/C9H10O2/c1-8(10)11-7-9-5-3-2-4-6-9/h2-6H,7H2,1H3
Specific details on test material used for the study:
- Name of test material (as cited in study report): Benzyl acetate
- Molecular formula: C9H10O2
- Molecular weight: 150.176 g/mol
- Substance type: organic
- Physical state: Liquid
- Smiles notation: c1(COC(C)=O)ccccc1
- InChl: 1S/C9H10O2/c1-8(10)11-7-9-5-3-2-4-6-9/h2-6H,7H2,1H3

Test animals / tissue source

Species:
rabbit
Strain:
other: albino
Details on test animals or tissues and environmental conditions:
No data available

Test system

Vehicle:
other: alcohol SDA 39
Controls:
yes
Amount / concentration applied:
0.1-mL aliquot of 3%, 7.5% or 18.7% benzyl acetate in alcohol SDA 39
Duration of treatment / exposure:
24 hours
Observation period (in vivo):
The observation period was once every 24 h for the first 4 days and at day seven.
Number of animals or in vitro replicates:
3 rabbits
Details on study design:
The left eye of the rabbits served as the control.

SCORING SYSTEM: The eyes were scored according to the Draize scale

Results and discussion

In vitro

Other effects / acceptance of results:
no data available

In vivo

Resultsopen allclose all
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
7 d
Reversibility:
not fully reversible within: 10 days
Remarks on result:
positive indication of irritation
Remarks:
7.5% in Alcohol SDA 39
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
7 d
Reversibility:
fully reversible within: 10 days
Remarks on result:
positive indication of irritation
Remarks:
3% in Alcohol SDA 39
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
7 d
Reversibility:
fully reversible within: 10 days
Remarks on result:
positive indication of irritation
Remarks:
18.7% in Alcohol SDA 39
Irritant / corrosive response data:
Using alcohol SDA 39 as the vehicle, conjunctival irritation was seen in all rabbits and corneal effects in one in three rabbits of each study. By the end of the 10th day the irritation had cleared at 3% and 18.7% but not at 7.5%.

Any other information on results incl. tables

Table: Results of the eye irritation study in rabbits

Dose(%)

Vehicle

Reactions

3

Alcohol SDA 39

Conjunctival irritation in all rabbits, corneal effects in 1 of 3 rabbits

7.5

Alcohol SDA 39

Conjunctival irritation in all rabbits, corneal effects in 1 of 3 rabbits

18.7

Alcohol SDA 39

Conjunctival irritation in all rabbits, corneal effects in 1 of 3 rabbits

Applicant's summary and conclusion

Interpretation of results:
Category 2 (irritating to eyes) based on GHS criteria
Conclusions:
Using alcohol SDA 39 as the vehicle, conjunctival irritation was seen in all rabbits and corneal effects in one in three rabbits of each study. By the end of the 10th day the irritation had cleared at 3% and 18.7% but not at 7.5%.
Hence,Benzyl acetate can be considered to be irritating to rabbit eyes
Executive summary:

An eye irritation study was conducted on chemical benzyl acetate. It was exposed to right eye of 3 albino rabbits for 24 hours using a 0.1-mL aliquot of 3%, 7.5% or 18.7%

benzyl acetate in alcohol SDA 39.The left eye of the rabbits served as the control. The observation period was once every 24 h for the first 4 days

and at day seven. The eyes were scored according to the Draize scale. Using alcohol SDA 39 as the vehicle, conjunctival irritation was seen in all rabbits and corneal effects in one in three rabbits of each study. By the end of the 10th day the irritation had cleared at 3% and 18.7% but not at 7.5%. Hence,Benzyl acetate can be considered to be irritating to rabbit eyes