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For the aquatic compartment two chronic key studies are available not for Triamine C16 -18 but for Triamine octadecenyl. The results of the Triamine octadecenyl will be used for Triamine C16 -18 as a worst-case as the octadecenyl will be more bioavailable than the C16 -18. For Triamine octadecenyl, the chronic algae test gave a ErC10 of 0.518 mg/L and the chronic daphnia test a NOEC (reproduction) of 0.81 mg/L. For the effect assessment the EC10 for daphnia adult mortalilty of 0.577 mg/L will be used as a worst-case. Note that both chronic key studies are higher tier studies performed in river water with relatively low (realistic worst-case) DOC and suspended matter concentrations. This means that for the risk assessment the bulk approach should be applied i.e. both the effect and exposure assessment are based on the total concentration (dissolved and sorbed).

For the toxicity to micro-organisms to Triamine C16 -18 an EC10 and EC50 of resp. 40 and 197 mg/L were observed after 3 h exposure.

For a comparison; For the toxicity to micro-organisms to Triamine octadecenyl an EC10 and EC50 of resp 18 and 115 mg/L were observed after 3 h exposure support the use of triamine octadecenyl data for read across for triamine C16 -18.