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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions (not tested in TA 1535, TA 1537 and TA 102; no data about purity of the test substance or cytotoxic effects)

Data source

Reference
Reference Type:
publication
Title:
Mutagenicity of dichloroacetylene and its degradation products trichloroacetyl chloride, trichloroacryloyl chloride and hexachlorobutadiene
Author:
Reichert D, Neudecker T, Spengler U & Henschler D
Year:
1983
Bibliographic source:
Mutat. Res. 117: 21-29

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Deviations:
yes
Remarks:
- modified test with vapour exposure; only 2 Salmonella strains were used instead of 5 (TA 1535, TA 1537 and TA 102 were not investigated)
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Phosgene
EC Number:
200-870-3
EC Name:
Phosgene
Cas Number:
75-44-5
Molecular formula:
CCl2O
IUPAC Name:
phosgene
Details on test material:
Phosgene were obtained from Messer Griesheim (Düsseldorf, Germany); no further data

Method

Species / strain
Species / strain / cell type:
other: Salmonella typhimurium TA 98, TA 100
Additional strain / cell type characteristics:
not applicable
Metabolic activation:
with and without
Metabolic activation system:
Aroclor 1254 induced male rat liver S9 mix
Test concentrations with justification for top dose:
Not specified, but unchanged phosgene was detected in the incubation medium by GC analysis only above a gaseous concentration of 10,000 ppm.
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
yes
True negative controls:
not specified
Positive controls:
yes
Positive control substance:
other: see remarks
Remarks:
the sensivities of the tester strains were routinely checked with 2-aminoanthracene as a standard mutagen in the presence of S9 mix and with 4-nitro-o-phenylenediamine (TA 98) or sodium azide (TA 100) in its absence
Statistics:
Not specified.

Results and discussion

Test results
Species / strain:
other: Salmonella typhimurium TA 98, TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not examined
Positive controls validity:
not specified
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

No specific mutagenicity results are reported for phosgene. However, the authors state: "Phosgene is non-mutagenic under the conditions of the S. typhimurium test system, because it reacts rapidly in the test medium. We detected unchanged phosgene in the solution only above a gaseous concentration of 10,000 ppm."

Applicant's summary and conclusion

Executive summary:

In a modified Ames test with vapour exposure of the test substance phosgene revealed no mutagenic activity with the Salmonella typhimurium strains TA 98 and TA 100 in the absence and in the presence of a metabolic activation system (Reichert et al., 1983). As explained by the authors phosgene would not be mutagenic under the conditions of liquid suspension in the Ames test, because this volatile gas reacts rapidly and completely with the components of the incubation system. Unchanged phosgene in the solution was only detected above a gaseous phosgene concentration of 10,000 ppm.