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Short-term toxicity to fish

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Description of key information

Fish 96 h-LC50: 0.73 mg/L

Key value for chemical safety assessment

LC50 for freshwater fish:
0.73 mg/L

Additional information

In the Klimisch 1 GLP study from Kimmel (2013) the acute toxicity of Sulfosuccinic (FA C16-18/18:1)E, Na/NH4 to zebra fish (Danio rerio) was determined in a 96‑hour semi-static test with daily test medium renewal according to the EU Commission Directive 92/69/, Part C.1, the Commission Regulation (EC) No 440/2008, Part C.1 and the OECD Guideline for Testing of Chemicals No. 203 (1992).

The nominal test item concentrations tested were 0.050, 0.16, 0.50, 1.6 and 5.0 mg/L. Additionally, a control group was tested in parallel. The concentrations of 0.050 and 0.16 mg/L were below the NOEC and hence not analysed. In the analyzed test medium samples from the start of the test medium renewal periods the measured test item concentrations ranged between 97 and 110% of the nominal values. This shows the correct dosage of the test item. During the test medium renewal periods of 24 hours a decrease of the test item concentration in the test media was determined. At the end of the first test medium renewal period, 70 to 83% of the nominal values were found. At the end of the last test medium renewal period, the measured concentration was below LOQ (limit of quantification, LOQ=0.301 mg test item/L). Therefore the biological results were related to the mean measured test item concentrations, i.e., control, 0.36, 1.5 and 4.1 mg/L for the control and nominal 0.50, 1.6 and 5.0 mg/L, respectively.

In the control and at the test concentrations up to and including the mean measured concentration of 0.36 mg/L, all fish survived until the end of the test and no visible abnormalities were observed in the test fish. At the next higher mean measured concentration of 1.5 mg/L, all test fish were dead after 48 hours of test duration. At the highest mean measured concentration of 4.1 mg/L, all fish were dead already after 24 hours of test duration.

The 96‑hour LC50 of Sulfosuccinic (FA C16-18/18:1)E, Na/NH4 was calculated to be 0.73 mg/L with a 95% confidence interval of 0.36 -1.5 mg/L. The 96‑hour LC100 was 1.5 mg/L.

The results of this study are considered relevant and reliable for the risk assessment.

Read across is made from CAS 147993-66 -6 (source) to the substance related to CAS 94213-67-9 (target). Both substances have a similar C-chain distribution. Assessment factors are not needed.