Reaction product of mixed inorganic base and acid resulting in complex boron, potassium and fluoride constituents in powder form.

Toxicity to aquatic plants other than algae

Administrative data

Endpoint:

toxicity to aquatic plants other than algae

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Peer-reviewed technical publication that meets basic scientific principles.

Data source

Reference

Reference Type:

publication

Title:

Toxicity Tests of Aquatic Pollutants by Using Common Duckweeed

Author:

Wang, W

Year:

1986

Bibliographic source:

Environmental Pollution (Series B) 11 (1986) 1-14

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Six different concentrations of boron were added to glass jars with duckweed and growth was recorded.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Boric acid

EC Number:

233-139-2

EC Name:

Boric acid

Cas Number:

10043-35-3

Molecular formula:

H3BO3

IUPAC Name:

Boric acid

Details on test material:

Chemical test compounds were reagent grade.

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Lemna minor

Details on test organisms:

TEST ORGANISM
- Common name: Duckweed
- Source (laboratory, culture collection): collected from a pond located inside the Illinois State Water Survey property during the spring of 1982
- Method of cultivation: The stock was maintained in a polypropylene tray with 20 litre capacity. To the tray was added a small amount of sediment, collected from the same pond, 10 litres of pond water and 5 litres of cold tap water. Every 3-4 days cold tap water was added to maintain the water level.
- 24 h before testing duckweed samples were selected for tests. The selected specimens must appear healthy, with 2 fronds of +- equal size per colony

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

7 d

Test conditions

Test temperature:

27 +- 2 °C

pH:

7.5

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 200 ml fruit jars, to each jar was added a toxicant and 200 ml of plant nutrient solution
- No. of colonies per vessel: 20
- No. of fronds per colony: 2
- No. of vessels per concentration (replicates): 3 - 6
- No. of vessels per control (replicates): 3 - 6


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: nutrient solution prepared according to Standard Methods (1980) by using deionised water


OTHER TEST CONDITIONS
- Light intensity and quality: cool-white fluorescent light with an intensity of 6456 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of frond number: manual counting

Results and discussion

Effect concentrations

Duration:

7 d

Dose descriptor:

NOEC

Effect conc.:

>= 60 mg/L

Nominal / measured:

nominal

Conc. based on:

element

Basis for effect:

frond number

Any other information on results incl. tables

7d EC50 growth >60 mg B/L