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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP, guideline study, adequate for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Di-n-butene. According to the report's owners the substance di-n-butene can be classified as CAS 11071-47-9 (Isooctene).
Components: 18 % n-Octene, 58 % Methylheptene and 24 % Dimethylhexene

Test animals / tissue source

Species:
rabbit
Strain:
other: small, white Russian (Chbb-SPF)
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 2.2
- Housing: stainless steel cages
- Diet (e.g. ad libitum): K4 Alleindiat for rabbits
- Water (e.g. ad libitum): tapwater
- Acclimation period: 14 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 ± 1
- Humidity (%): 60 ± 5
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12 / 12

Test system

Vehicle:
unchanged (no vehicle)
Controls:
no
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 cm3


Duration of treatment / exposure:
72 hours
Observation period (in vivo):
1, 24, 48 and 72 hours after application and also at 6 days after application
Number of animals or in vitro replicates:
3
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): saline solution
- Time after start of exposure: 72 hours


SCORING SYSTEM: Draize


TOOL USED TO ASSESS SCORE: hand-slit lamp and fluorescein

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 72 hours
Score:
0
Max. score:
0
Reversibility:
other: not relevant
Remarks on result:
other: score also 0 at 6 days
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 72 hours
Score:
0
Max. score:
0
Reversibility:
other: not relevant
Remarks on result:
other: score also 0 at 6 days
Irritation parameter:
conjunctivae score
Remarks:
redness
Basis:
mean
Time point:
other: 72 hours
Score:
0
Max. score:
0
Reversibility:
other: not relevant
Remarks on result:
other: score also 0 at 6 days
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 72 hours
Score:
0
Max. score:
0
Reversibility:
other: not relevant
Remarks on result:
other: score also 0 at 6 days

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
Under the test conditions, di-n-butene was not irritating to rabbit eyes