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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: screening test, other
Remarks:
BOD5/COD
Type of information:
other: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Study period:
June 19,1979
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Short summary
Remarks:
The test was conducted by means of Read Across approach. The reliability of the source study report is 4. Further information was attached at section 13
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
not specified
Qualifier:
equivalent or similar to
Guideline:
EU Method C.6 (Degradation: Chemical Oxygen Demand)
Deviations:
not specified
GLP compliance:
no
Remarks:
Pre GLP.
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
ca. 5 d
Initial conc.:
ca. 500 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Parameter:
% degradation (O2 consumption)
Remarks on result:
not measured/tested
Parameter:
BOD5
Value:
ca. 0 mg O2/g test mat.
Parameter:
COD
Value:
ca. 994.36 mg O2/g test mat.
Parameter:
BOD5*100/COD
Value:
ca. 0 mg O2/g test mat.

Biochemical oxygen demand or BOD is the amount of dissolved oxygen needed by aerobic biological organisms in a body of water to break down organic material present in a given water sample at certain temperature over a specific time period. The term also refers to a chemical procedure for determining this amount. This is not a precise quantitative test, although it is widely used as an indication of the organic quality of water, therefore is not be used as quantitative date about ready biodegradation.

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
BOD5 = 0 mg/g O2
COD = 994.36 mg/g O2
BOD5/COD = 0
Executive summary:

BOD5 = 0 mg/g O2

COD = 994.36 mg/g O2

BOD5/COD = 0

Endpoint:
biodegradation in water: screening test, other
Remarks:
BOD5/COD
Type of information:
other: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Study period:
October 31, 1977
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Short summary
Remarks:
The test was conducted by means of Read Across approach. The reliability of the source study report is 4. Further information was attached at section 13
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
not specified
Qualifier:
equivalent or similar to
Guideline:
EU Method C.6 (Degradation: Chemical Oxygen Demand)
Deviations:
not specified
GLP compliance:
no
Remarks:
Pre GLP.
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
ca. 5 d
Initial conc.:
ca. 50 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Parameter:
% degradation (O2 consumption)
Remarks on result:
not measured/tested
Parameter:
BOD5
Value:
ca. 108 mg O2/g test mat.
Parameter:
COD
Value:
ca. 919.94 mg O2/g test mat.
Parameter:
BOD5*100/COD
Value:
ca. 12 mg O2/g test mat.

Biochemical oxygen demand or BOD is the amount of dissolved oxygen needed by aerobic biological organisms in a body of water to break down organic material present in a given water sample at certain temperature over a specific time period. The term also refers to a chemical procedure for determining this amount. This is not a precise quantitative test, although it is widely used as an indication of the organic quality of water, therefore is not be used as quantitative date about ready biodegradation.

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
BOD5=108 mg/g O2
COD=919.94 mg/g O2
BOD5/COD=0.12
Executive summary:

BOD5=108 mg/g O2

COD=919.94 mg/g O2

BOD5/COD=0.12

Endpoint:
biodegradation in water: screening test, other
Remarks:
BOD5/COD
Type of information:
other: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Study period:
November 7,1978
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
abstract
Remarks:
The test was conducted by means of Read Across approach. The reliability of the source study report is 4. Further information was attached at section 13
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
not specified
Qualifier:
equivalent or similar to
Guideline:
EU Method C.6 (Degradation: Chemical Oxygen Demand)
Deviations:
not specified
GLP compliance:
no
Remarks:
Pre GLP.
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
ca. 5 d
Initial conc.:
ca. 50 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Parameter:
% degradation (O2 consumption)
Remarks on result:
not measured/tested
Parameter:
BOD5
Value:
ca. 116 mg O2/g test mat.
Parameter:
COD
Value:
ca. 992.29 mg O2/g test mat.
Parameter:
BOD5*100/COD
Value:
ca. 11.69 mg O2/g test mat.

Biochemical oxygen demand or BOD is the amount of dissolved oxygen needed by aerobic biological organisms in a body of water to break down organic material present in a given water sample at certain temperature over a specific time period. The term also refers to a chemical procedure for determining this amount. This is not a precise quantitative test, although it is widely used as an indication of the organic quality of water, therefore is not be used as quantitative date about ready biodegradation.

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
BOD5 = 116 mg/g O2
COD = 992.29 mg/g O2
BOD5/COD = 0.12
Executive summary:

BOD5 = 116 mg/g O2

COD = 992.29 mg/g O2

BOD5/COD = 0.12

Endpoint:
biodegradation in water: screening test, other
Remarks:
BOD5
Type of information:
other: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Study period:
From July 5 to 10, 1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
The test was conducted by means of Read Across approach. The reliability of the source study report is 1. Further information was attached at section 13
Qualifier:
according to
Guideline:
other: EEC Directive 79-831, Annex V, Part C.8 , DEGRADATION BIOCHEMICAL OXYGEN DEMAND
Deviations:
no
Qualifier:
according to
Guideline:
other: according to DEV H-5 (a2) Deutsche Einheitsverfahren, 1966
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.9 (Biodegradation: Zahn-Wellens Test)
Deviations:
no
Qualifier:
according to
Guideline:
other: German DIN-Norm 38'409, H-41, 1
Deviations:
no
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge:mixed culture of bacteria (effluent of a Husman laboratory apparatus)
Duration of test (contact time):
ca. 5 d
Initial conc.:
ca. 1 mg/L
Based on:
test mat.
Initial conc.:
ca. 5 mg/L
Based on:
test mat.
Initial conc.:
ca. 10 mg/L
Based on:
test mat.
Initial conc.:
ca. 20 mg/L
Based on:
test mat.
Initial conc.:
ca. 50 mg/L
Based on:
test mat.
Initial conc.:
ca. 100 mg/L
Based on:
test mat.
Initial conc.:
ca. 200 mg/L
Based on:
test mat.
Initial conc.:
ca. 500 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Test temperature: 22 ± 2°C

TEST SYSTEM
- Culturing apparatus:
- Number of culture flask: 1
- Measuring equipment: O2 Determination with an Oxygensensitive-electrode: SYLAND

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
Reference substance:
benzoic acid, sodium salt
Parameter:
% degradation (O2 consumption)
Value:
ca. 0
Sampling time:
5 d
Parameter:
BOD5
Value:
ca. 0 mg O2/g test mat.
Parameter:
COD
Value:
ca. 992 mg O2/g test mat.
Parameter:
BOD5*100/COD
Value:
0
Results with reference substance:
for 4 mg/l reference substance = 1050 mgO2/g
for 5 mg/l reference substance = 1060 mgO2/g

VALIDITY CRITERIA:

- Blank

B0D5: 0,5 or less mgO2/l

- Reference substance

B0D5: not less than 960 mgO2/l

(concentration 4 mg/l and 5 mg/l)

Test substance:

Test concentration mg/l O2-Start O2-End Blank BOD5
mgO2/g
1 8.9 8.4 0.2 300
5 8.9 8.5 0.2 40
10 8.9 8.5 0.2 20
20 8.9 8.3 0.2 20
50 8.9 8.4 0.2 6
100 8.9 8.4 0.2 3
200 8.9 8.4 0.2 2
500 8.8 8.3 0.2 1

Reference substance:

Test concentration mg/l O2-Start O2-End Blank BOD5
mgO2/g
4 8.8 4.4 0.2 1050
5 8.9 3.4 0.2 1060

The B0D5 of the test item can not be calculated because the measured end value "02 -End" is below 2 mgO2/l and

therefore it does not fulfil the validity criteria.

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The BOD5 of the test substance, corrected by the blank control is: 0 mgO2/g, therefore BOD5/COD = 0
Executive summary:

The substance has been tested for ready biodegradation according to the EEC Directive 79-831, Annex V, Part. C.8 and according to DEV H-5 (a2) Deutsche Einheitsverfahren, 1966. The value of degradation of test item is below the limit of detection, therefore under test conditions no biodegradation it is observed.

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
other: read-across from supporting substance (structural analogue or surrogate)read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Study period:
From August 2 to September 12, 1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
The test was conducted by means of Read Across approach. The reliability of the source study report is 1. Further information was attached at section 13
Qualifier:
according to
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Deviations:
no
Qualifier:
according to
Guideline:
other: ISO Method 8129 B
Deviations:
no
GLP compliance:
yes (incl. certificate)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, adapted
Details on inoculum:
- Source of inoculum/activated sludge: 1000 mg/l
- Laboratory culture: Sludge of a domestic sewage treatment plant (ARA-Rhein) collected on 14.08.90
Duration of test (contact time):
ca. 28 d
Initial conc.:
ca. 100 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Composition of medium: According to the specification of the EEC L133 (pp.99-105) Volume 31 resp. OECD Method 302B.
- Test temperature:22 ± 3 °C
- Continuous darkness: no, indirect daylight


Reference substance:
diethylene glycol
Parameter:
% degradation (O2 consumption)
Value:
ca. 62.45
Sampling time:
28 d
Details on results:
Adsorption after 3 hours: 31.35 %
Results with reference substance:
Biodegradation corrected by the blank control and measured as DOC (mg/l) is: 99.6 % (12th day)

Biodegradation after 28 days:

TIME Value of the substance DOC (mg/l) Value of the blank DOC (mg/l) Elimination (%)
1° sample 2° sample 1° sample 2° sample 1° sample 2° sample
0 h 78.2 79.5 6 6 24.7 23.8
3 h 73.9 72.6 7.2 7.2 30.5 32.2
2 day 62 65.5 4.4 4.4 39.9 36.7
5 day 59.9 59.1 7.4 7.4 45.3 46.4
7 day 58.9 58.1 5.6 5.6 44.4 45.6
9 day 59.1 58.3 8.1 8.1 46.8 48
12 day 56.9 56.8 8.9 8.9 50 50.4
14 day 58.1 55.6 7.5 7.5 47.2 50.2
16 day 54.3 52.9 11 11 54.9 56.6
19 day 51.6 48.8 10.9 10.9 57.6 60.7
21 day 53.2 48.4 12.1 12.1 57.1 62.4
23 day 49.8 49.5 10.1 10.1 58.6 59.2
26 day 48.7 46.7 9.5 9.5 59.1 61.5
28 day 47.7 46.9 11.2 11 .2 61.9 63.0

Reference substance:

TIME Value of the substance DOC (mg/l) Value of the blank DOC (mg/l) Elimination (%)
0 h 107.6 6 0
3 h 105.9 7.2 0.3
2 day 107.2 4.4 0
5 day 15.8 7.4 91 .5
6 day 4.9 5.3 100
7 day 5.7 5.6 99.9
8 day 7.9 6.3 98.4
9 day 8.4 8.1 99.7
12 day 9.3 8.9 99.6

Validity criteria:

The test is considered valid if the procedural control shows the removal of the reference compound by at least 70 % within 14d and if the removal of DOC (or COD) in the test suspension took place relatively gradually over days or weeks, since this indicates biodegradation.

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
Elimination measured as DOC (mg/l) after 28 days corrected by the blank control is: 62.45 %
Adsorption after 3 hours = 31.35 %
Executive summary:

The substance has been tested for Inherent biodegradation according to the OECD 302B. The biodegradation after 28 days , corrected by the blank control, it is: 62.45 % (average of 2 tests running in parallel). Adsorption after 3 hours = 31.35 %.

Description of key information

Elimination = 62.45 %

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable

Additional information