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EC number: 701-122-3 | CAS number: 106185-75-5
Acute Immobilisation of Daphnids after 24 and 48 Hours in the Final Test:
An acute aquatic toxicity study was performed on Daphnia magna in accordance with GLP and OECD Guideline 202. Following a preliminary range-finding test, twenty daphnids (four replicates, five daphnids/replicate) were exposed to an aqueous solution of the test item, at the required nominal test concentrations of 0.5, 0.8, 1.3, 2.0, 3.2 and 5 mg/L. The immobility of the daphnids was determined in a semi-static 48h test by visual observation after 24 and 48 h. The concentrations of the test item were determined by chemical analyses at the start (t = 0 h), at t = 24 h (new and old solutions) and at the end of the test (t = 48 h). Although concentrations measured in freshly prepared solutions and in 24-hour old solutions revealed that the concentration of the test item was satisfactorily maintained within ± 20% of the initial concentration during the first day, losses of ca. 30% were recorded during the second exposure period (t=24hFresh-t=48h) at 1.3 and 3.2 mg/L (nominal).Therefore, since the deviation of the exposure concentrations of the test substance was greater than 20% of the initial concentrations only for two test concentrations, it was decided to express the biological results in terms of geometric means of the exposure concentrations for the present study: 0.48, 0.70, 1.13, 1.82, 3.15, and 4.86 mg/L. After 24 hours of exposure, immobilisations were 0% at 0.48, 0.70 and 1.13 mg/L 10% at 1.82 mg/L, 75% at 3.15 mg/L and 100% at 4.86 mg test item/L. After 48 hours of exposure, immobilisations were 0% at 0.48 and 0.70 mg/L, 35% at 1.13 mg/L, 80% at 1.82 mg/L and 100% at 3.15 and 4.86 mg test item/L. The EC50 at each of the observation times was 2.59 mg/L (24h) and 1.34 mg/L (48h).
This toxicity study is considered acceptable and satisfies the guideline requirement for acute daphnid toxicity study.
The substance exhibits an EC50 for freshwater invertebrates of 1.34 mg/L.
Two experimental data and one QSAR data are available to determine the short-term toxicity to aquatic invertebrates.
The 48–hr acute toxicity of 2-ethyl-4-(2,2,3-trimethyl-3-cyclopenten-1-yl)-(2E)-buten-1-ol to Daphnia magna was studied under semi-static conditions. Daphnids were exposed to the test chemical at nominal test concentrations of 0, 0.5, 0.8, 1.3, 2.0, 3.2, 5.0 mg/L for 48 h. Immobility of daphnids was determined by visual observation after 24 and 48 hours. Concentrations of the test item were determined by chemical analyses at the start (t=0h), at t=24h (new and old solutions) and at the end of the test (t=48h). After 48 hours of exposure, immobilisation rates were 0% at 0, 0.48 and 0.70 mg/L, 35% at 1.13 mg/L, 80% at 1.82 mg/L, 100% at 3.15 mg/L and 4.86 mg/L (measured concentrations). The calculated 48–hour EC50 was 1.34 mg/L. Based on the results of this study, 2-ethyl-4-(2,2,3-trimethyl-3-cyclopenten-1-yl)-(2E)-buten-1-ol has to be classified as hazardous to the aquatic environment (chronic 2 – H411 Toxic to aquatic life with long lasting effects) in accordance with the EU classification system EU.
This toxicity study is considered as acceptable and satisfies the guideline requirement for acute daphnid toxicity study.
This study is given a reliability score of 1 (reliable without restrictions) since the test method meets the requirement of the EU test method and the OECD test guidelines with consideration of the “difficult to test” nature of the test substance.
In an acute aquatic toxicity study performed according to the EPA OPPTS 850.1010 guideline, groups of Daphnia magna were exposed to Super Radjanol at test concentrations of 0, 0.65, 1.3, 2.5, 5.0 and 10.0 mg/L (nominal) for 48 hours under flow-through conditions. Mobility/mortality was observed after 48 hours.
Mobility/mortality (48 hours) at 0, 0.38, 0.80, 1.7, 3.4 or 8.1 mg/L (measured) were 0, 0, 5, 75, 100 and 100%, respectively. Calculated EC50 at 24 and 48 hours were 2.6 and 1.4 mg/L respectively, based on the measured concentrations of the test item.
This toxicity study is classified as acceptable and satisfies the guideline requirements for an acute daphnid toxicity study.
This study is given a reliability score of 2 (reliable with restrictions) since the test method is considered as an alternative to the EU test method and the OECD test guideline.
A QSAR prediction was performed to assess the acute toxicity of the test item 2-ethyl-4-(2,2,3-trimethyl-3-cyclopenten-1-yl)-(2E)-buten-1-ol to daphnid. This QSAR has been validated to be compliant with the OECD recommendations for QSAR modeling (OECD, 2004) and predicts the endpoint value which would be expected when testing the substance under experimental conditions in a laboratory following Guideline for Testing of Chemicals No. 202, "Daphniasp., Acute Immobilisation Test" (1), referenced as Method C.2 of Commission Regulation No. 440/2008 (2). The criterion predicted was the EC50(Median Effective Concentration), a statistically derived concentration which is expected to cause immobility in 50% of test animals within a period of 48 hours.
The QSAR determination of the daphnids immobility was based on validated data derived from 48-hour tests on daphnids, for which the concentrations of the test item had been determined by chemical analyses over the test periods.
The 48h-EC50 was predicted as 1.6 mg/L. This toxicity study is considered acceptable and satisfies the guideline requirements for acute daphnid toxicity study.
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