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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
14 - 18 Jan 1991
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report Date:
1991

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DIN 38412/L15
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Leuciscus idus melanotus
Details on test organisms:
TEST ORGANISM
- Common name: golden orfe
- Source: Fischtzucht Eggers, Hohenweststedt, Germany
- Age at study initiation (mean and range, SD): consigned on 13 Nov 1990
- Length at study initiation (length definition, mean, range and SD): 7.4 cm (6.7 - 8.4 cm)
- Weight at study initiation (mean and range, SD): 4.0 g (3.0 - 5.5 g)

ACCLIMATION
- Acclimation period: 2 months
- Acclimation conditions (same as test or not): same
- Health during acclimation (any mortality observed): 0.06% mortality during last two weeks of housing

FEEDING DURING TEST
- Food type: no feeding during test

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
2.5 mmol/L
Test temperature:
20°C
pH:
7.7 - 9.1
Dissolved oxygen:
7.5 - 8.6 mg/L
Nominal and measured concentrations:
10000 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel: glass aquarium
- Material, size, headspace, fill volume: 30x22x24 cm, 10 L
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 4 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: demineralized tap water, resalted to reconstituted water according to DIN 38412/11
- Ca/mg ratio: 4:1
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 light / 8 darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, sublethal symptoms

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 1000 mg/L
- Results used to determine the conditions for the definitive study: yes (mortality 1/5)
Reference substance (positive control):
yes
Remarks:
chloroacetamide

Results and discussion

Effect concentrations
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: none
- Observations on body length and weight: none
- Other biological observations: none
- Mortality of control: 0%
- Other adverse effects control: 1 fish died after 24 h
Results with reference substance (positive control):
48-h LC50: 32 mg/L (1991-03-25)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 96-h LC50 was determined to be >10000 mg/L. The test substance is not acutely toxic to fish.