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Diss Factsheets
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EC number: 204-479-9 | CAS number: 121-54-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jaunary 1986
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 870.2500 (Acute Dermal Irritation)
- Deviations:
- no
- Principles of method if other than guideline:
- DOT Skin Corrosion, Department of Transportation CFR Title 49, 173, 1200
- GLP compliance:
- no
Test material
- Reference substance name:
- Benzethonium chloride
- EC Number:
- 204-479-9
- EC Name:
- Benzethonium chloride
- Cas Number:
- 121-54-0
- Molecular formula:
- C27H42NO2.Cl
- IUPAC Name:
- benzyldimethyl(2-{2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethoxy}ethyl)azanium chloride
- Test material form:
- solid: compact
Constituent 1
- Specific details on test material used for the study:
- - Source and lot/batch No.of test material: Lonza Inc., Fair Lawn, New Jersey
- product name: HYAMINE 1622 Powder (K-11-16)
- description: white powder
- Expiration date of the lot/batch: no data
- Purity test date: no data
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Albino Rabbits from Davidson's Mill Farm, USA
- Age at study initiation: no data
- Weight at study initiation: no data
- Housing: animals were individually housed in stainless steel wire bottomed cages
- Diet (e.g. ad libitum): Purina Rabbit Pellets
- Water (e.g. ad libitum): water ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): room temperature
- Photoperiod (hrs dark / hrs light): 12 hour light/dark cycle
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- clipped
- Vehicle:
- water
- Controls:
- no
- Amount / concentration applied:
- 0.78 grams of test material was uniformly mixed with deionized distilled water and applied as a 63.7% suspension (w/w) to give 0.5 grams of material per site
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- Reading 4 and 48 hours as well as 5 and 8 days after exposure
- Number of animals:
- 6 animals
- Details on study design:
- A 2.5 cm2 gauze patch was placed over intact skin on each rabbit. Five-tenths of a gram of the testmaterial was placed under each patch. The patches were secured in place with adhesive tape. The entire trunk of each animal was wrapped with a rubberized elastic cloth to retard evaporation and as an aid in maintaining test patch position. The rabbits were then immobilized in head stocks for 4 hours after which time the patches were removed. All test sites were washed to prevent further exposure. Corrosion readings were made at 4 and 48 hours after exposure. Corrosion was considered to have resulted if the test substance caused destruction or irreversible alteration of the tissue. Tissue destruction was considered to have occurred if, at any of the readings, there was ulceration or necrosis. Epidermal sloughing, erythema, edema or fissuring were not considered tissue destruction.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- other:
- Basis:
- mean
- Time point:
- other: 4 h
- Remarks on result:
- probability of weak irritation
- Irritation parameter:
- other:
- Basis:
- mean
- Time point:
- 48 h
- Remarks on result:
- positive indication of irritation
- Irritation parameter:
- other:
- Basis:
- mean
- Time point:
- other: 8 d
- Remarks on result:
- positive indication of irritation
- Irritant / corrosive response data:
- At 4 hours the test site was brownish in color. Thickening of the test site with reduced flexibility was alsp noted. There was no evidence of necrosis, ulceration or sloughing. At 48 hours one rabbit exhibited necrosis. The test sites on the other rabbits remained brown (slightly darker than at 4 hours) and thickened, with reduced flexibility. At 8 days the rabbits exhibited necrosis, however, there were no signs of ulceration or sloughing. In conclusion, the test item needs to be considered as corrosive to rabbit skin.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1B (corrosive) based on GHS criteria
- Conclusions:
- Based on the 4 and 48 hour scores, the test material is considered to be corrosive to the skin.
- Executive summary:
Skin corrosion was tested in accordance with DOT Skin Corrosion, Department of Transportation CFR Title 49, 173, 1200. Six NZW rabbits were treated occlusive with 0.78 g of test item mixed with deionized water for 4 hours. Skin reactions were assessed 4 hours, 48 hours and 8 days after removal of the patch. At 4 hours the test site was brownish in color. Thickening of the test site with reduced flexibility was alsp noted. There was no evidence of necrosis, ulceration or sloughing. At 48 hours one rabbit exhibited necrosis. The test sites on the other rabbits remained brown (slightly darker than at 4 hours) and thickened, with reduced flexibility. At 8 days the rabbits exhibited necrosis, however, there were no signs of ulceration or sloughing. Based on the 4 and 48 hour scores, the test material is considered to be corrosive to the skin.
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