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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: screening test, other
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1977
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Qualifier:
no guideline available
Principles of method if other than guideline:
- Principle of test: Biochemical oxygen demand determined after 5 d at 20°C inoculation
- Parameters analysed / observed: oxygen content at 0 h and after 5 d inoculation
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial (adaptation not specified)
Duration of test (contact time):
5 d
Parameter:
% degradation (O2 consumption)
Remarks on result:
other: BOD5
Parameter:
BOD5
Value:
150 mg O2/g test mat.
Remarks on result:
other: Test material concentration: 10 mg/L
Parameter:
COD
Value:
656 mg O2/g test mat.
Remarks on result:
other: Test material concentration: 5 mg/L
Key result
Parameter:
BOD5*100/COD
Value:
23 mg O2/g test mat.
Validity criteria fulfilled:
not applicable
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The BSB5/COD ratio is 0.23. Therefore the substance is not considered to be readily biodegradable.
Endpoint:
biodegradation in water: screening test, other
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1977
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
significant methodological deficiencies
Qualifier:
no guideline available
Principles of method if other than guideline:
- Principle of test: Biochemical oxygen demand determined after 5 d at 20°C inoculation
- Parameters analysed / observed: oxygen content at 0 h and after 5 d inoculation
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial (adaptation not specified)
Duration of test (contact time):
5 d
Parameter:
% degradation (O2 consumption)
Remarks on result:
other: BOD5
Key result
Parameter:
BOD5
Value:
180 mg O2/g test mat.
Remarks on result:
other: Test material concentration: 10 mg/L
Parameter:
COD
Value:
973 mg O2/g test mat.
Remarks on result:
other: Test material concentration: 5 mg/L
Validity criteria fulfilled:
not applicable
Interpretation of results:
not readily biodegradable
Conclusions:
Since BOD5 and COD were measured with different test material concentrations, the level of biodegradation cannot be interpreted accurately. However, the BSB5/COD ratio is <1. Therefore the substance is not considered to be readily biodegradable.
Endpoint:
biodegradation in water: screening test, other
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1978
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
significant methodological deficiencies
Qualifier:
no guideline available
Principles of method if other than guideline:
- Principle of test: Biochemical oxygen demand determined after 5 d at 20°C inoculation
- Parameters analysed / observed: oxygen content at 0 h and after 5 d inoculation
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial (adaptation not specified)
Duration of test (contact time):
5 d
Parameter:
% degradation (O2 consumption)
Remarks on result:
other: BOD5
Key result
Parameter:
BOD5
Value:
31 mg O2/g test mat.
Remarks on result:
other: Test material concentration: 100 mg/L
Parameter:
COD
Value:
1 627 mg O2/g test mat.
Remarks on result:
other: Test material concentration: 5 mg/L
Validity criteria fulfilled:
not applicable
Interpretation of results:
not readily biodegradable
Conclusions:
Since BOD5 and COD were measured with different test material concentrations, the level of biodegradation cannot be interpreted accurately. However, the BSB5/COD ratio is <1. Therefore the substance is not considered to be readily biodegradable.
Endpoint:
biodegradation in water: screening test, other
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1978
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
significant methodological deficiencies
Qualifier:
no guideline available
Principles of method if other than guideline:
- Principle of test: Biochemical oxygen demand determined after 5 d at 20°C inoculation
- Parameters analysed / observed: oxygen content at 0 h and after 5 d inoculation
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial (adaptation not specified)
Duration of test (contact time):
5 d
Parameter:
% degradation (O2 consumption)
Remarks on result:
other: BOD5
Key result
Parameter:
BOD5
Value:
115 mg O2/g test mat.
Remarks on result:
other: Test material concentration: 40 mg/L
Parameter:
COD
Value:
924 mg O2/g test mat.
Remarks on result:
other: Test material concentration: 5 mg/L
Validity criteria fulfilled:
not applicable
Interpretation of results:
not readily biodegradable
Conclusions:
Since BOD5 and COD were measured with different test material concentrations, the level of biodegradation cannot be interpreted accurately. However, the BSB5/COD ratio is <1. Therefore the substance is not considered to be readily biodegradable.
Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1977
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Remarks:
No information given on negative control and validity criteria.
Qualifier:
according to guideline
Guideline:
other: Modified OECD Confirmatory Tests
Version / remarks:
The test was conducted according to SOP A-12 (1974-09-01).
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial (adaptation not specified)
Duration of test (contact time):
31 d
Initial conc.:
20 mg/L
Based on:
TOC
Initial conc.:
58 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
TOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium: TOC content of medium: 107 mg/L

TEST SYSTEM
- Number of culture flasks/concentration: 1


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
Key result
Parameter:
% degradation (TOC removal)
Value:
98
St. dev.:
4
Sampling time:
21 d
Parameter:
BOD5
Value:
175 mg O2/g test mat.
Remarks on result:
other: Test item conc. 20 mg/L
Parameter:
COD
Value:
874 mg O2/g test mat.
Remarks on result:
other: Test item conc. 10 mg/L
Validity criteria fulfilled:
not specified
Interpretation of results:
inherently biodegradable
Conclusions:
The substance was found to be inherently biodegradable (98% after 21 d based on TOC removal).
Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1978
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Remarks:
No information given on negative control and validity criteria.
Qualifier:
according to guideline
Guideline:
other: Modified OECD Confirmatory Tests
Version / remarks:
The test was conducted according to SOP A-12 (1974-09-01).
GLP compliance:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial (adaptation not specified)
Duration of test (contact time):
21 d
Initial conc.:
20 mg/L
Based on:
TOC
Initial conc.:
57.9 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
TOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium: TOC content of medium: 110 mg/L

TEST SYSTEM
- Number of culture flasks/concentration: 1


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
Key result
Parameter:
% degradation (TOC removal)
Value:
84
St. dev.:
3
Sampling time:
21 d
Parameter:
BOD5
Value:
35 mg O2/g test mat.
Remarks on result:
other: Test item conc. 100 mg/L
Parameter:
COD
Value:
1 759 mg O2/g test mat.
Remarks on result:
other: Test item conc. 5 mg/L
Validity criteria fulfilled:
not specified
Interpretation of results:
inherently biodegradable
Conclusions:
The substance was found to be inherently biodegradable (84% after 21 d based on TOC removal).

Description of key information

Based on a weight-of-evidence approach with two inherent biodegradability tests and several BOD5/COD determinations, the test substance can be considered inherently biodegradable. The substance is not readily biodegradable according to CLP criteria.

Key value for chemical safety assessment

Biodegradation in water:
inherently biodegradable

Additional information